Inhibition of human cytotoxic T lymphocyte activity in vitro by autologous peripheral blood granulocytes

H. T. Petrie, L. W. Klassen, H. D. Kay

Research output: Contribution to journalArticle

146 Scopus citations

Abstract

Peripheral blood granulocytes from normal healthy donors were found to reproducibly inhibit the cytolytic effector function of specifically sensitized cytotoxic T lymphocytes in vitro when coincubated with these effector cells and target cells in 8 hr 51Cr release assays. Inhibition required intact granulocytes, was proportional to the number of granulocytes present, and was independent of granulocyte adherence, phagocytic function, and viability. Equivalent numbers of enriched normal or leukemic peripheral T lymphocytes did not cause inhibition of 51Cr release, and preincubation of granulocytes with effectors did not significantly alter viability or cytotoxic function. Because granulocytes can inhibit natural killer cell function in vitro, these data indicate that granulocytes can regulate diverse antigen-specific and spontaneous cytotoxic function in vitro, suggesting that circulating granulocytes may have the potential for in vivo regulation of these cytotoxic effectors.

Original languageEnglish (US)
Pages (from-to)230-234
Number of pages5
JournalJournal of Immunology
Volume134
Issue number1
StatePublished - 1985

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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