TY - JOUR
T1 - Inhibition of lens epithelial cell growth by induction of apoptosis
T2 - Potential for prevention of posterior capsule opacification
AU - Geissler, Francis T.
AU - Cheng Li, David Wan
AU - James, Eric R.
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2001/11/1
Y1 - 2001/11/1
N2 - As a model of the cell proliferation occurring in posterior capsule opacification (PCO), lens epithelial cells (LEC) from human and rabbit capsulotomies, and a rabbit LEC line (N/N1003A) were grown in Dulbecco’s Minimal Essential Media (MEM) with 10% fetal calf serum. LEC were exposed to the calcium ionophore, calcimycin, and viability was assessed by trypan blue staining, growth by 3H-thymidine incorporation and apoptosis by annexin/propidium iodide staining, calcein AM/ethidium bromide staining and DNA laddering. Human capsulotomy samples were similarly exposed to calcimycin, and apoptosis assayed by calcein AM/ethidium bromide staining. Calcimycin exposure induced apoptosis in both rabbit LEC cultures and human LEC, and changes leading to apoptosis could be detected within 30 minutes of calcimycin treatment. The decrease in viability and growth in human and rabbit LEC was dose-dependent. These data support the further evaluation of apoptosis induction as a possible treatment mechanism to prevent development of PCO following primary cataract surgery in humans.
AB - As a model of the cell proliferation occurring in posterior capsule opacification (PCO), lens epithelial cells (LEC) from human and rabbit capsulotomies, and a rabbit LEC line (N/N1003A) were grown in Dulbecco’s Minimal Essential Media (MEM) with 10% fetal calf serum. LEC were exposed to the calcium ionophore, calcimycin, and viability was assessed by trypan blue staining, growth by 3H-thymidine incorporation and apoptosis by annexin/propidium iodide staining, calcein AM/ethidium bromide staining and DNA laddering. Human capsulotomy samples were similarly exposed to calcimycin, and apoptosis assayed by calcein AM/ethidium bromide staining. Calcimycin exposure induced apoptosis in both rabbit LEC cultures and human LEC, and changes leading to apoptosis could be detected within 30 minutes of calcimycin treatment. The decrease in viability and growth in human and rabbit LEC was dose-dependent. These data support the further evaluation of apoptosis induction as a possible treatment mechanism to prevent development of PCO following primary cataract surgery in humans.
UR - http://www.scopus.com/inward/record.url?scp=0035672239&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035672239&partnerID=8YFLogxK
U2 - 10.1089/10807680152729275
DO - 10.1089/10807680152729275
M3 - Article
C2 - 11777182
AN - SCOPUS:0035672239
VL - 17
SP - 587
EP - 596
JO - Journal of Ocular Pharmacology
JF - Journal of Ocular Pharmacology
SN - 1080-7683
IS - 6
ER -