Inhibition of miR-328-3p impairs cancer stem cell function and prevents metastasis in ovarian cancer

Amit K. Srivastava, Ananya Banerjee, Tiantian Cui, Chunhua Han, Shurui Cai, Lu Liu, Dayong Wu, Ri Cui, Zaibo Li, Xiaoli Zhang, Guozhen Xie, Karuppaiyah Selvendiran, Srinivas Patnaik, Adam R. Karpf, Jinsong Liu, David E. Cohn, Qi En Wang

Research output: Contribution to journalArticlepeer-review

64 Scopus citations


Cancer stem cells (CSC) play a central role in cancer metastasis and development of drug resistance.miRNAare important in regulating CSC properties and are considered potential therapeutic targets. Herewereport that miR-328-3p (miR-328) is significantly upregulated in ovarian CSC. High expression of miR-328 maintained CSC properties by directly targeting DNA damage binding protein 2,which has been shownpreviously to inhibit ovarian CSC. Reduced activity of ERK signaling in ovarian CSC, mainly due to a low level of reactive oxygen species, contributed to the enhanced expression of miR-328 and maintenance of CSC. Inhibition of miR-328 in mouse orthotopic ovarian xenografts impeded tumor growth and prevented tumor metastasis. In summary, our findings provide a novel mechanism underlying maintenance of the CSC population in ovarian cancer and suggest that targeted inhibition of miR-328 could be exploited for the eradication of CSC and aversion of tumor metastasis in ovarian cancer. Significance: These findings present inhibition of miR-328 as a novel strategy for efficient elimination of CSC to prevent tumor metastasis and recurrence in patients with epithelial ovarian cancer.

Original languageEnglish (US)
Pages (from-to)2314-2326
Number of pages13
JournalCancer Research
Issue number9
StatePublished - 2019

ASJC Scopus subject areas

  • Oncology
  • Cancer Research


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