Inhibition of protein kinase C epsilon causes ciliated bovine bronchial cell detachment

Rebecca E. Slager, Joseph H. Sisson, Jaqueline A. Pavlik, Jennifer K. Johnson, Jeremy R. Nicolarsen, Thomas R. Jerrells, Todd A. Wyatt

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

This study defines the in vitro phenomenon of ciliated bovine bronchial epithelial cell (BBEC) detachment from the basal epithelium and regulation of cilia motility mediated through protein kinase C epsilon (PKCε). The authors determined the time course of activation and downregulation of PKCε by the known PKC activator phorbol 12-myristate 13-acetate (PMA) and demonstrate that chemical inhibition of PKC by calphostin C or the novel PKC isoform inhibitor Ro 31-8220 induced striking detachment of ciliated BBECs from the basal cell monolayer within 1 hour, independent of apoptosis or necrotic cell death. The results of this study support a possible novel PKCε-mediated signaling pathway through which ciliated cell attachment is maintained.

Original languageEnglish (US)
Pages (from-to)349-362
Number of pages14
JournalExperimental Lung Research
Volume32
Issue number8
DOIs
StatePublished - Sep 1 2006

Keywords

  • Airway epithelium
  • Bronchial epithelial cell
  • Cell detachment
  • Cilia
  • Kinase
  • Protein kinase C epsilon

ASJC Scopus subject areas

  • Molecular Biology
  • Pulmonary and Respiratory Medicine
  • Clinical Biochemistry

Fingerprint Dive into the research topics of 'Inhibition of protein kinase C epsilon causes ciliated bovine bronchial cell detachment'. Together they form a unique fingerprint.

  • Cite this