Inhibition of protein kinase C epsilon causes ciliated bovine bronchial cell detachment

Rebecca E. Slager; Joseph H. Sisson; Jaqueline A. Pavlik; Jennifer K. Johnson; Jeremy R. Nicolarsen; Thomas R. Jerrells; Todd A. Wyatt

doi:10.1080/01902140600959630

Inhibition of protein kinase C epsilon causes ciliated bovine bronchial cell detachment

Rebecca E. Slager, Joseph H. Sisson, Jaqueline A. Pavlik, Jennifer K. Johnson, Jeremy R. Nicolarsen, Thomas R. Jerrells, Todd A. Wyatt

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

This study defines the in vitro phenomenon of ciliated bovine bronchial epithelial cell (BBEC) detachment from the basal epithelium and regulation of cilia motility mediated through protein kinase C epsilon (PKCε). The authors determined the time course of activation and downregulation of PKCε by the known PKC activator phorbol 12-myristate 13-acetate (PMA) and demonstrate that chemical inhibition of PKC by calphostin C or the novel PKC isoform inhibitor Ro 31-8220 induced striking detachment of ciliated BBECs from the basal cell monolayer within 1 hour, independent of apoptosis or necrotic cell death. The results of this study support a possible novel PKCε-mediated signaling pathway through which ciliated cell attachment is maintained.

Original language	English (US)
Pages (from-to)	349-362
Number of pages	14
Journal	Experimental Lung Research
Volume	32
Issue number	8
DOIs	https://doi.org/10.1080/01902140600959630
State	Published - Sep 1 2006

Keywords

Airway epithelium
Bronchial epithelial cell
Cell detachment
Cilia
Kinase
Protein kinase C epsilon

ASJC Scopus subject areas

Molecular Biology
Pulmonary and Respiratory Medicine
Clinical Biochemistry

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10.1080/01902140600959630

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title = "Inhibition of protein kinase C epsilon causes ciliated bovine bronchial cell detachment",

abstract = "This study defines the in vitro phenomenon of ciliated bovine bronchial epithelial cell (BBEC) detachment from the basal epithelium and regulation of cilia motility mediated through protein kinase C epsilon (PKCε). The authors determined the time course of activation and downregulation of PKCε by the known PKC activator phorbol 12-myristate 13-acetate (PMA) and demonstrate that chemical inhibition of PKC by calphostin C or the novel PKC isoform inhibitor Ro 31-8220 induced striking detachment of ciliated BBECs from the basal cell monolayer within 1 hour, independent of apoptosis or necrotic cell death. The results of this study support a possible novel PKCε-mediated signaling pathway through which ciliated cell attachment is maintained.",

keywords = "Airway epithelium, Bronchial epithelial cell, Cell detachment, Cilia, Kinase, Protein kinase C epsilon",

author = "Slager, {Rebecca E.} and Sisson, {Joseph H.} and Pavlik, {Jaqueline A.} and Johnson, {Jennifer K.} and Nicolarsen, {Jeremy R.} and Jerrells, {Thomas R.} and Wyatt, {Todd A.}",

note = "Funding Information: This research was supported by VA Merit Review (TAW), NRSA Institutional Fellowship 5T32AA007582 (RES), and NIH R01 AA008769 (JHS). The authors wish to thank Lisa Chudomelka for manuscript preparation and Jan Taylor of the UNMC Laser Scanning Microscope Core Facility for operation of the confocal microscope.",

year = "2006",

month = sep,

day = "1",

doi = "10.1080/01902140600959630",

language = "English (US)",

volume = "32",

pages = "349--362",

journal = "Experimental Lung Research",

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T1 - Inhibition of protein kinase C epsilon causes ciliated bovine bronchial cell detachment

AU - Slager, Rebecca E.

AU - Sisson, Joseph H.

AU - Pavlik, Jaqueline A.

AU - Johnson, Jennifer K.

AU - Nicolarsen, Jeremy R.

AU - Jerrells, Thomas R.

AU - Wyatt, Todd A.

N1 - Funding Information: This research was supported by VA Merit Review (TAW), NRSA Institutional Fellowship 5T32AA007582 (RES), and NIH R01 AA008769 (JHS). The authors wish to thank Lisa Chudomelka for manuscript preparation and Jan Taylor of the UNMC Laser Scanning Microscope Core Facility for operation of the confocal microscope.

PY - 2006/9/1

Y1 - 2006/9/1

N2 - This study defines the in vitro phenomenon of ciliated bovine bronchial epithelial cell (BBEC) detachment from the basal epithelium and regulation of cilia motility mediated through protein kinase C epsilon (PKCε). The authors determined the time course of activation and downregulation of PKCε by the known PKC activator phorbol 12-myristate 13-acetate (PMA) and demonstrate that chemical inhibition of PKC by calphostin C or the novel PKC isoform inhibitor Ro 31-8220 induced striking detachment of ciliated BBECs from the basal cell monolayer within 1 hour, independent of apoptosis or necrotic cell death. The results of this study support a possible novel PKCε-mediated signaling pathway through which ciliated cell attachment is maintained.

AB - This study defines the in vitro phenomenon of ciliated bovine bronchial epithelial cell (BBEC) detachment from the basal epithelium and regulation of cilia motility mediated through protein kinase C epsilon (PKCε). The authors determined the time course of activation and downregulation of PKCε by the known PKC activator phorbol 12-myristate 13-acetate (PMA) and demonstrate that chemical inhibition of PKC by calphostin C or the novel PKC isoform inhibitor Ro 31-8220 induced striking detachment of ciliated BBECs from the basal cell monolayer within 1 hour, independent of apoptosis or necrotic cell death. The results of this study support a possible novel PKCε-mediated signaling pathway through which ciliated cell attachment is maintained.

KW - Airway epithelium

KW - Bronchial epithelial cell

KW - Cell detachment

KW - Cilia

KW - Kinase

KW - Protein kinase C epsilon

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DO - 10.1080/01902140600959630

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SN - 0190-2148

VL - 32

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EP - 362

JO - Experimental Lung Research

JF - Experimental Lung Research

IS - 8

ER -

Inhibition of protein kinase C epsilon causes ciliated bovine bronchial cell detachment

Abstract

Keywords

ASJC Scopus subject areas

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