Abstract
This study defines the in vitro phenomenon of ciliated bovine bronchial epithelial cell (BBEC) detachment from the basal epithelium and regulation of cilia motility mediated through protein kinase C epsilon (PKCε). The authors determined the time course of activation and downregulation of PKCε by the known PKC activator phorbol 12-myristate 13-acetate (PMA) and demonstrate that chemical inhibition of PKC by calphostin C or the novel PKC isoform inhibitor Ro 31-8220 induced striking detachment of ciliated BBECs from the basal cell monolayer within 1 hour, independent of apoptosis or necrotic cell death. The results of this study support a possible novel PKCε-mediated signaling pathway through which ciliated cell attachment is maintained.
Original language | English (US) |
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Pages (from-to) | 349-362 |
Number of pages | 14 |
Journal | Experimental Lung Research |
Volume | 32 |
Issue number | 8 |
DOIs | |
State | Published - Sep 1 2006 |
Keywords
- Airway epithelium
- Bronchial epithelial cell
- Cell detachment
- Cilia
- Kinase
- Protein kinase C epsilon
ASJC Scopus subject areas
- Molecular Biology
- Pulmonary and Respiratory Medicine
- Clinical Biochemistry