Initiation and characterization of cultures of human colonic carcinoma with different biological characteristics utilizing feeder layers of confluent fibroblasts

M. G. Brattain, D. E. Brattain, W. D. Fine, F. M. Khaled, M. E. Marks, P. M. Kimball, L. A. Arcolano, B. H. Danbury

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Abstract

The tissue culture of human colonic carcinoma with and without feeder layers of confluent C3H 10T 1/2 mouse fibroblasts was compared. In a series of 27 different tumor specimens, 21 long-term cultures (6 mos. or more) were obtained by utilizing feeder layers. Only 3 long-term cultures were obtained from the same set of specimens when feeder layers were not employed. Several of the long-term cultures were established as cell lines weaned from fibroblast feeder layers. These lines could be classified into 3 groups based upon their expression of several biological properties including tumorigenicity in nude mice, degree of differentiation of the tumors growing in nude mice, growth in semisolid medium, morphology in vitro and production of carcinoembryonic antigen in vitro.

Original languageEnglish (US)
Pages (from-to)355-366
Number of pages12
JournalOncodevelopmental Biology and Medicine
Volume2
Issue number5
StatePublished - 1981

ASJC Scopus subject areas

  • Medicine(all)

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    Brattain, M. G., Brattain, D. E., Fine, W. D., Khaled, F. M., Marks, M. E., Kimball, P. M., Arcolano, L. A., & Danbury, B. H. (1981). Initiation and characterization of cultures of human colonic carcinoma with different biological characteristics utilizing feeder layers of confluent fibroblasts. Oncodevelopmental Biology and Medicine, 2(5), 355-366.