TY - JOUR
T1 - Insights regarding fungal phosphoproteomic analysis
AU - Ribeiro, Liliane F.C.
AU - Chelius, Cynthia L.
AU - Harris, Steven D.
AU - Marten, Mark R.
N1 - Funding Information:
This work was supported by the National Science Foundation [grant numbers 1517309 & 1601935]. Any opinions, findings, and conclusions or recommendations expressed in this material are those of the authors and do not necessarily reflect the views of the National Science Foundation.
Publisher Copyright:
© 2017 Elsevier Inc.
PY - 2017/7/1
Y1 - 2017/7/1
N2 - Protein phosphorylation is a major means of regulation for cellular processes, and is important in cell signaling, growth, and cell proliferation. To study phosphorylated proteins, high throughput phosphoproteomic technologies, such as reverse phase protein array, phospho-specific flow cytometry, and mass spectrometry (MS) based technologies, have been developed. Among them, mass spectrometry has become the primary tool employed for the identification of phosphoproteins and phosphosites in fungi, leading to an improved understanding of a number of signaling pathways. Using mass spectrometry techniques, researchers have discovered new kinase substrates, established connections between kinases and fungal pathogenicity, and studied the evolutionary lineage of kinases between different fungal species. Further, many specific phosphorylation sites recognized by individual kinases have been described. In this review, we will focus on recent discoveries made in yeast and filamentous fungi using phosphoproteomic analysis.
AB - Protein phosphorylation is a major means of regulation for cellular processes, and is important in cell signaling, growth, and cell proliferation. To study phosphorylated proteins, high throughput phosphoproteomic technologies, such as reverse phase protein array, phospho-specific flow cytometry, and mass spectrometry (MS) based technologies, have been developed. Among them, mass spectrometry has become the primary tool employed for the identification of phosphoproteins and phosphosites in fungi, leading to an improved understanding of a number of signaling pathways. Using mass spectrometry techniques, researchers have discovered new kinase substrates, established connections between kinases and fungal pathogenicity, and studied the evolutionary lineage of kinases between different fungal species. Further, many specific phosphorylation sites recognized by individual kinases have been described. In this review, we will focus on recent discoveries made in yeast and filamentous fungi using phosphoproteomic analysis.
KW - Cellular regulation
KW - Filamentous fungi
KW - Mass spectrometry
KW - Phosphorylation
KW - Yeast
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U2 - 10.1016/j.fgb.2017.03.003
DO - 10.1016/j.fgb.2017.03.003
M3 - Review article
C2 - 28288883
AN - SCOPUS:85018457530
VL - 104
SP - 38
EP - 44
JO - Fungal Genetics and Biology
JF - Fungal Genetics and Biology
SN - 1087-1845
ER -