Insulin rapidly increases diacylglycerol by activating de novo phosphatidic acid synthesis

Robert V. Farese, Thomas Suman Konda, John S. Davis, Mary L. Standaert, Robert J. Pollet, Denise R. Cooper

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Abstract

The mechanisms whereby insulin increases diacylglycerol in BC3H-1 myocytes were examined. When [3H]arachidonate labeling of phospholipids was used as an indicator of phospholipase C activation, transient increases in [3H]diacylglycerol were observed between 0.5 and 10 minutes after the onset of insulin treatment. With [3H]glycerol labeling as an indicator of de novo phospholipid synthesis, [3H]diacylglycerol was increased maximally at 1 minute and remained elevated for 20 minutes. [ 3H]Glycerol-labeled diacylglycerol was largely derived directly from phosphatidic acid. Insulin increased de novo phosphatidic acid synthesis within 5 to 10 seconds; within 1 minute, this synthesis was 60 times greater than that of controls. Thus, the initial increase in diacylglycerol is due to both increased hydrolysis of phospholipids and a burst of de novo phosphatidic acid synthesis. After 5 to 10 minutes, de novo phosphatidic acid synthesis continues as a major source of diacylglycerol. Both phospholipid effects of insulin seem important for generating diacylglycerol and other phospholipid-derived intracellular signaling substances.

Original languageEnglish (US)
Pages (from-to)586-589
Number of pages4
JournalScience
Volume236
Issue number4801
DOIs
StatePublished - Jan 1 1987

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Farese, R. V., Konda, T. S., Davis, J. S., Standaert, M. L., Pollet, R. J., & Cooper, D. R. (1987). Insulin rapidly increases diacylglycerol by activating de novo phosphatidic acid synthesis. Science, 236(4801), 586-589. https://doi.org/10.1126/science.3107122