Interaction of gangliosides with B cells in splenic fragment cultures

William G. Chaney, Harold C. Miller, Norman R. Klinman, Walter J. Esselman

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

The effect of gangliosides upon murine adult B cells at the single precursor cell level was examined using the splenic focus assay. Adult B cells were stimulated in in vitro organ fragment culture by a hapten-modified carrier protein in the presence of an excess of carrier-primed T cells. The addition of a potential tolerogen in the form of antigen coupled to a carrier not previously presented to the T cells resulted in a temporary unresponsiveness of the onset of antibody production in adult B cells, but not a permanent state of tolerance. This delay could be eliminated by the addition of purified murine gangliosides during the presentation of the hapten coupled to the unrecognized carrier. The ganglioside preparation was fractionated by ion-exchange chromatography and the active fraction was found to be a disialoganglioside. These results suggest that the ganglioside may interfere with membrane receptor-related events occurring during or after antigen binding or cross-linking to responding B cells.

Original languageEnglish (US)
Pages (from-to)165-170
Number of pages6
JournalCellular Immunology
Volume86
Issue number1
DOIs
StatePublished - Jun 1984

ASJC Scopus subject areas

  • Immunology

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    Chaney, W. G., Miller, H. C., Klinman, N. R., & Esselman, W. J. (1984). Interaction of gangliosides with B cells in splenic fragment cultures. Cellular Immunology, 86(1), 165-170. https://doi.org/10.1016/0008-8749(84)90369-1