Interaction of tRNA with domain II of 23S rRNA

Walter E. Hill; Anchalee Tassanakajohn; William E. Tapprich

doi:10.1016/0167-4781(90)90139-S

Interaction of tRNA with domain II of 23S rRNA

Walter E. Hill, Anchalee Tassanakajohn, William E. Tapprich

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

The interaction of tRNA with domain II of 23S rRNA in E. coli ribosomes has been probed using short, complementary DNA oligodeoxyribonucleotides. Specifically, cDNA oligomers to the region 801-811 of the 23S rRNA were used to ascertain the interaction of this region with tRNA. It was found that when tRNA was bound to the P site, considerable competition occurred between tRNA and the cDNA oligomers which base paired with the nucleotides 807-811. However, A-site bound tRNA neither displaced, nor was displaced, by cDNA oligomers to this region. Additionally, the binding of tRNA lacking the CACCA nucleotides on the 3′ terminus was unaffected by the presence a cDNA oligomer complementary to nucleotides 803-811, indicating that the cDNA-tRNA competition was dependent on the 3′ terminal nucleotides of tRNA.

Original language	English (US)
Pages (from-to)	45-50
Number of pages	6
Journal	BBA - Gene Structure and Expression
Volume	1050
Issue number	1-3
DOIs	https://doi.org/10.1016/0167-4781(90)90139-S
State	Published - Aug 27 1990
Externally published	Yes

Keywords

Domain II
rRNA, 23S
tRNA binding

ASJC Scopus subject areas

Structural Biology
Biophysics
Biochemistry
Genetics

Access to Document

10.1016/0167-4781(90)90139-S

Cite this

@article{f55ea7165e0543babebb27de61d0c555,

title = "Interaction of tRNA with domain II of 23S rRNA",

abstract = "The interaction of tRNA with domain II of 23S rRNA in E. coli ribosomes has been probed using short, complementary DNA oligodeoxyribonucleotides. Specifically, cDNA oligomers to the region 801-811 of the 23S rRNA were used to ascertain the interaction of this region with tRNA. It was found that when tRNA was bound to the P site, considerable competition occurred between tRNA and the cDNA oligomers which base paired with the nucleotides 807-811. However, A-site bound tRNA neither displaced, nor was displaced, by cDNA oligomers to this region. Additionally, the binding of tRNA lacking the CACCA nucleotides on the 3′ terminus was unaffected by the presence a cDNA oligomer complementary to nucleotides 803-811, indicating that the cDNA-tRNA competition was dependent on the 3′ terminal nucleotides of tRNA.",

keywords = "Domain II, rRNA, 23S, tRNA binding",

author = "Hill, {Walter E.} and Anchalee Tassanakajohn and Tapprich, {William E.}",

year = "1990",

month = aug,

day = "27",

doi = "10.1016/0167-4781(90)90139-S",

language = "English (US)",

volume = "1050",

pages = "45--50",

journal = "BBA - Gene Structure and Expression",

issn = "0167-4781",

publisher = "Elsevier B.V.",

number = "1-3",

}

TY - JOUR

T1 - Interaction of tRNA with domain II of 23S rRNA

AU - Hill, Walter E.

AU - Tassanakajohn, Anchalee

AU - Tapprich, William E.

PY - 1990/8/27

Y1 - 1990/8/27

N2 - The interaction of tRNA with domain II of 23S rRNA in E. coli ribosomes has been probed using short, complementary DNA oligodeoxyribonucleotides. Specifically, cDNA oligomers to the region 801-811 of the 23S rRNA were used to ascertain the interaction of this region with tRNA. It was found that when tRNA was bound to the P site, considerable competition occurred between tRNA and the cDNA oligomers which base paired with the nucleotides 807-811. However, A-site bound tRNA neither displaced, nor was displaced, by cDNA oligomers to this region. Additionally, the binding of tRNA lacking the CACCA nucleotides on the 3′ terminus was unaffected by the presence a cDNA oligomer complementary to nucleotides 803-811, indicating that the cDNA-tRNA competition was dependent on the 3′ terminal nucleotides of tRNA.

AB - The interaction of tRNA with domain II of 23S rRNA in E. coli ribosomes has been probed using short, complementary DNA oligodeoxyribonucleotides. Specifically, cDNA oligomers to the region 801-811 of the 23S rRNA were used to ascertain the interaction of this region with tRNA. It was found that when tRNA was bound to the P site, considerable competition occurred between tRNA and the cDNA oligomers which base paired with the nucleotides 807-811. However, A-site bound tRNA neither displaced, nor was displaced, by cDNA oligomers to this region. Additionally, the binding of tRNA lacking the CACCA nucleotides on the 3′ terminus was unaffected by the presence a cDNA oligomer complementary to nucleotides 803-811, indicating that the cDNA-tRNA competition was dependent on the 3′ terminal nucleotides of tRNA.

KW - Domain II

KW - rRNA, 23S

KW - tRNA binding

UR - http://www.scopus.com/inward/record.url?scp=0025118160&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025118160&partnerID=8YFLogxK

U2 - 10.1016/0167-4781(90)90139-S

DO - 10.1016/0167-4781(90)90139-S

M3 - Article

C2 - 2169894

AN - SCOPUS:0025118160

SN - 0167-4781

VL - 1050

SP - 45

EP - 50

JO - BBA - Gene Structure and Expression

JF - BBA - Gene Structure and Expression

IS - 1-3

ER -

Interaction of tRNA with domain II of 23S rRNA

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this