Interleukin-1β, but not interleukin-6, enhances renal and systemic endothelin production in vivo

Erika I. Boesen, Jennifer M. Sasser, Mohamed A. Saleh, William A. Potter, Mandy Woods, Timothy D. Warner, Jennifer S. Pollock, David M. Pollock

Research output: Contribution to journalArticlepeer-review

35 Scopus citations


The inflammatory cytokines IL-1β and IL-6 have been shown to stimulate production of endothelin-1 (ET-1) by several cell types in vitro, but their effects on renal ET-1 production in vivo are not known. To test whether IL-1β and IL-6 stimulate renal ET-1 production and release in vivo, urine was collected from male C57BL/6 mice over 24-h periods at baseline and on days 7 and 14 of a 14-day subcutaneous infusion of IL-1β (10 ng/h), IL-6 (16 ng/h), or vehicle. By day 14, plasma ET-1 was significantly increased by IL-1β infusion (1.7 ± 0.1 vs. 0.8 ± 0.1 pg/ml for vehicle, P < 0.001). Compared with vehicle infusion, IL-1β infusion induced significant increases in urinary ET-1 excretion rate and urine flow but did not affect conscious mean arterial pressure (telemetry). IL-1β infusion significantly increased renal cortical and medullary IL-1β content (ELISA) and prepro-ET-1 mRNA expression (quantitative real-time PCR). In contrast, 14 days of IL-6 infusion had no significant effect on plasma ET-1 or urinary ET-1 excretion rate. To determine whether IL-1β stimulates ET-1 release via activation of NF-κB, inner medullary collecting duct (IMCD-3) cells were incubated for 24 h with IL-1β, and ET-1 release and NF-κB activation were measured (ELISA). IL-1β activated NF-κB and increased ET-1 release in a concentration-dependent manner. The effect of IL-1β on ET-1 release could be partially inhibited by pretreatment of IMCD-3 cells with an inhibitor of NF-κB activation (BAY 11-7082). These results indicate that IL-1β stimulates renal and systemic ET-1 production in vivo, providing further evidence that ET-1 participates in inflammatory responses.

Original languageEnglish (US)
Pages (from-to)F446-F453
JournalAmerican Journal of Physiology - Renal Physiology
Issue number2
StatePublished - Aug 2008
Externally publishedYes


  • Inflammatory cytokines
  • Mouse inner medullary collecting duct cell line
  • Nuclear factor-κB
  • Renal inflammatory conditions

ASJC Scopus subject areas

  • Physiology
  • Urology


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