Interleukin-2-induced chemotaxis of human T-lymphocytes

R. A. Robbins, L. Klassen, J. Rasmussen, M. E. Clayton, W. D. Russ

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Interleukin-2 (IL-2), a growth factor for T-lymphocytes, has been postulated to cause the accumulation of T-lymphocytes at sites of inflammation by inducing proliferation of these cells. We hypothesized that IL-2 might also serve to attract T-lymphocytes to inflammatory sites. To test this hypothesis, human T-lymphocytes were purified from the peripheral blood of normal volunteers by rosetting with neuraminidase-treated sheep red blood cells and tested for chemotactic activity by using a blind-well chamber technique. Purified IL-2 caused a greater than 20-fold attraction of T-lymphocytes compared with medium alone (P < 0.001). This attraction was shown to be chemotactic rather than chemokinetic by checkerboard analysis. The T-lymphocyte chemotaxis could be completely inhibited by adsorption of the IL-2 with an IL-2-dependent cell line, and could be neutralized by monoclonal anti-IL-2 antibody. Further specificity of IL-2-directed chemotaxis was demonstrated by using species-specific IL-2. Mouse IL-2 was ineffective at promoting human T-lymphocyte chemotaxis. These data suggest that IL-2 may be responsible for the localized accumulation of T-lymphocytes both by causing attraction of these cells and by modulating their proliferation.

Original languageEnglish (US)
Pages (from-to)340-345
Number of pages6
JournalJournal of Laboratory and Clinical Medicine
Issue number4
StatePublished - 1986

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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    Robbins, R. A., Klassen, L., Rasmussen, J., Clayton, M. E., & Russ, W. D. (1986). Interleukin-2-induced chemotaxis of human T-lymphocytes. Journal of Laboratory and Clinical Medicine, 108(4), 340-345.