TY - JOUR
T1 - Interspecies and intraspecies signals synergistically regulate lysobacter enzymogenes twitching motility
AU - Feng, Tao
AU - Han, Yong
AU - Li, Bingqing
AU - Li, Zhiqiang
AU - Yu, Yameng
AU - Sun, Qingyang
AU - Li, Xiaoyu
AU - Du, Liangcheng
AU - Zhang, Xiao Hua
AU - Wang, Yan
N1 - Funding Information:
This work was supported by the National Natural Science Foundation of China (no. 31870023, 31571970, and 41506160), the Fundamental Research Funds for the Central Universities (no. 201941009), the Young Elite Scientists Sponsorship Program by CAST (no. YESS20160009), and the Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao) (no. 2018SDKJ0406-4). Y.W. and Y.H. conceived the project. Y.W., L.D., and Y.H. designed the experiments. T.F., Y.H., B.L., Z.L., Y.Y., X.L., and Q.S. carried out experiments. Y.W., T.F., and Y.H. analyzed the data. Y.W. wrote the manuscript draft. L.D. and T.F. revised the manuscript. All of us read and approved the submission for publication. We declare that we have no conflicts of interest.
Publisher Copyright:
© 2019 American Society for Microbiology.
PY - 2019/12/1
Y1 - 2019/12/1
N2 - The twitching motility of bacteria is closely related to environmental adaptability and pathogenic behaviors. Lysobacter is a good genus in which to study twitching motility because of the complex social activities and distinct movement patterns of its members. Regardless, the mechanism that induces twitching motility is largely unknown. In this study, we found that the interspecies signal indole caused Lysobacter to have irregular, random twitching motility with significantly enhanced speed. Deletion of qseC or qseB from the two-component system for indole signaling perception resulted in the disappearance of rapid, random movements and significantly decreased twitching activity. Indole-induced, rapid, random twitching was achieved through upregulation of expression of gene cluster pilE1-pilY11-pilX1- pilW1-pilV1-fimT1. In addition, under conditions of extremely low bacterial density, individual Lysobacter cells grew and divided in a stable manner in situ without any movement. The intraspecies quorum-sensing signaling factor 13-methyltetradecanoic acid, designated L. enzymogenes diffusible signaling factor (LeDSF), was essential for Lysobacter to produce twitching motility through indirect regulation of gene clusters pilM-pilN-pilO-pilP-pilQ and pilS1-pilR-pilA-pilB-pilC. These results demonstrate that the motility of Lysobacter is induced and regulated by indole and LeDSF, which reveals a novel theory for future studies of the mechanisms of bacterial twitching activities.
AB - The twitching motility of bacteria is closely related to environmental adaptability and pathogenic behaviors. Lysobacter is a good genus in which to study twitching motility because of the complex social activities and distinct movement patterns of its members. Regardless, the mechanism that induces twitching motility is largely unknown. In this study, we found that the interspecies signal indole caused Lysobacter to have irregular, random twitching motility with significantly enhanced speed. Deletion of qseC or qseB from the two-component system for indole signaling perception resulted in the disappearance of rapid, random movements and significantly decreased twitching activity. Indole-induced, rapid, random twitching was achieved through upregulation of expression of gene cluster pilE1-pilY11-pilX1- pilW1-pilV1-fimT1. In addition, under conditions of extremely low bacterial density, individual Lysobacter cells grew and divided in a stable manner in situ without any movement. The intraspecies quorum-sensing signaling factor 13-methyltetradecanoic acid, designated L. enzymogenes diffusible signaling factor (LeDSF), was essential for Lysobacter to produce twitching motility through indirect regulation of gene clusters pilM-pilN-pilO-pilP-pilQ and pilS1-pilR-pilA-pilB-pilC. These results demonstrate that the motility of Lysobacter is induced and regulated by indole and LeDSF, which reveals a novel theory for future studies of the mechanisms of bacterial twitching activities.
KW - Interspecies and intraspecies signal LeDSF
KW - Interspecies and intraspecies signal indole
KW - Lysobacter enzymogenes
KW - Quorum-sensing
KW - Twitching motility
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U2 - 10.1128/AEM.01742-19
DO - 10.1128/AEM.01742-19
M3 - Article
C2 - 31540995
AN - SCOPUS:85075090188
SN - 0099-2240
VL - 85
JO - Applied and environmental microbiology
JF - Applied and environmental microbiology
IS - 23
M1 - e01742-19
ER -