TY - JOUR
T1 - Interspecies variation in hominid gut microbiota controls host gene regulation
AU - Muehlbauer, Amanda L.
AU - Richards, Allison L.
AU - Alazizi, Adnan
AU - Burns, Michael B.
AU - Gomez, Andres
AU - Clayton, Jonathan B.
AU - Petrzelkova, Klara
AU - Cascardo, Camilla
AU - Resztak, Justyna
AU - Wen, Xiaoquan
AU - Pique-Regi, Roger
AU - Luca, Francesca
AU - Blekhman, Ran
N1 - Publisher Copyright:
© 2021 The Author(s)
PY - 2021/11/23
Y1 - 2021/11/23
N2 - The gut microbiome exhibits extreme compositional variation between hominid hosts. However, it is unclear how this variation impacts host physiology across species and whether this effect can be mediated through microbial regulation of host gene expression in interacting epithelial cells. Here, we characterize the transcriptional response of human colonic epithelial cells in vitro to live microbial communities extracted from humans, chimpanzees, gorillas, and orangutans. We find that most host genes exhibit a conserved response, whereby they respond similarly to the four hominid microbiomes. However, hundreds of host genes exhibit a divergent response, whereby they respond only to microbiomes from specific host species. Such genes are associated with intestinal diseases in humans, including inflammatory bowel disease and Crohn's disease. Last, we find that inflammation-associated microbial species regulate the expression of host genes previously associated with inflammatory bowel disease, suggesting health-related consequences for species-specific host-microbiome interactions across hominids.
AB - The gut microbiome exhibits extreme compositional variation between hominid hosts. However, it is unclear how this variation impacts host physiology across species and whether this effect can be mediated through microbial regulation of host gene expression in interacting epithelial cells. Here, we characterize the transcriptional response of human colonic epithelial cells in vitro to live microbial communities extracted from humans, chimpanzees, gorillas, and orangutans. We find that most host genes exhibit a conserved response, whereby they respond similarly to the four hominid microbiomes. However, hundreds of host genes exhibit a divergent response, whereby they respond only to microbiomes from specific host species. Such genes are associated with intestinal diseases in humans, including inflammatory bowel disease and Crohn's disease. Last, we find that inflammation-associated microbial species regulate the expression of host genes previously associated with inflammatory bowel disease, suggesting health-related consequences for species-specific host-microbiome interactions across hominids.
KW - Gut microbiome, Primates, Hominids, Gene regulation
UR - http://www.scopus.com/inward/record.url?scp=85119507935&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85119507935&partnerID=8YFLogxK
U2 - 10.1016/j.celrep.2021.110057
DO - 10.1016/j.celrep.2021.110057
M3 - Article
C2 - 34818542
AN - SCOPUS:85119507935
SN - 2211-1247
VL - 37
JO - Cell Reports
JF - Cell Reports
IS - 8
M1 - 110057
ER -