Intestinal neoplasia in the Apc(Min) mouse: Independence from the microbial and natural killer (beige locus) status

We have tested the hypothesis that enteric bacteria are necessary for formation of intestinal adenomas in C57BL/6-Apc(Min)/+ mouse. Germ-free mice developed 2-fold fewer adenomas than conventional controls in the medial small intestine (7.3 versus 14.9; P < 0.003), but there were no significant differences in the rest of the intestinal tract. We conclude that microbial status does not strongly alter the adenoma phenotype in this mouse model of familial adenomatous polyposis. In parallel, we have found that C57BL/6- Apc(MIN)/+ mice mutated at the beige locus, which controls natural killer activity, are also unaltered in adenoma multiplicity.