Intracellular localization and nucleocytoplasmic trafficking of steroid receptors: An overview

Sanjay Kumar, Mallampati Saradhi, Nagendra K. Chaturvedi, Rakesh K. Tyagi

Research output: Contribution to journalReview article

66 Scopus citations

Abstract

Subcellular compartmentalization and dynamic movements of steroid receptors are major steps in executing their transcription regulatory function. Though significant progress has been made in understanding the mechanisms underlying nuclear import of NLS-bearing proteins, our general and mechanistic understanding about the nuclear export processes has begun to emerge only recently. The discovery of most commonly utilized CRM1/exportin11 dependent nuclear export pathway is attributed to a potent nuclear export inhibitor leptomycin B that helped dissecting this and other nuclear export pathways. Simultaneously, utilization of green fluorescent protein (GFP)-tagged intracellular steroid receptors has contributed to not only resolving controversial issue of subcellular localization of unliganded hormone receptors but also provided further insight into finer details of receptor dynamics in living cells. With judicious use of leptomycin B and expression of GFP-tagged receptors in living cells, existence of exportin1/CRM1 independent pathway(s), nuclear export signals and receptors for bi-directional translocation that are unique to steroid receptor trafficking have been specified. Currently, we appear to be arriving at a consensus that steroid/nuclear receptors follow dynamic nucleocytoplasmic processes that deviate from the ones commonly utilized by majority of other proteins.

Original languageEnglish (US)
Pages (from-to)147-156
Number of pages10
JournalMolecular and Cellular Endocrinology
Volume246
Issue number1-2
DOIs
StatePublished - Feb 26 2006

Keywords

  • CRM1
  • Exportin1
  • Importin
  • Leptomycin
  • Nucleocytoplasmic shuttling
  • Steroid receptors

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology

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