Intraclot microbubble combined with urokinase mediated ultrasound thrombolysis: Experiment in vitro

Qiong Zhu, Shunji Gao, Mengjiao Guo, Yuan Gao, Zheng Liu, Feng Xie

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


Objective: To investigate the efficiency of intraclot microbubbles (MB) combined with urokinase (UK) mediated ultrasound (US) thrombolysis. Methods: Fifty clots prepared by bovine whole blood were equally divided into 5 groups and were treated in a circulation system with collateral circulation tube. The clots were treated by US, MB and UK in group 1, by US and MB in group 2, by US and UK in group 3 and by UK in group 4. The group 5 was the control group without any treatment. The thrombolysis rate of each group was measured and compared. Residual clots were histologically observed with hematoxylin-eosin staining and immunofluorescence. Results: The thrombolysis rate of group 1 ([73.64±14.16]%) was significantly higher than group 2 ([47.97±11.66]%), group 3 ([57.33±8.65]%), group 4 ([50.85±9.63]%), and group 5 ([29.76±18.06]%, all P<0.05). Histological examination of the clots in group 1 showed multiple thrombolysis foci with clots collapse, and the degradation of fibrin network was further confirmed by immunofluorescence. Conclusion: The intraclot MB mediated US thrombolysis combined with UK can enhance the rate of thrombolysis in vitro experiment.

Original languageEnglish (US)
Pages (from-to)242-246
Number of pages5
JournalChinese Journal of Interventional Imaging and Therapy
Issue number4
StatePublished - Apr 10 2017


  • Microbubbles
  • Thrombolysis
  • Thrombosis
  • Ultrasonics

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging


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