Intraclot microbubble combined with urokinase mediated ultrasound thrombolysis: Experiment in vitro

Qiong Zhu; Shunji Gao; Mengjiao Guo; Yuan Gao; Zheng Liu; Feng Xie

doi:10.13929/j.1672-8475.201611037

Intraclot microbubble combined with urokinase mediated ultrasound thrombolysis: Experiment in vitro

Qiong Zhu, Shunji Gao, Mengjiao Guo, Yuan Gao, Zheng Liu, Feng Xie

Cardiovascular Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

Objective: To investigate the efficiency of intraclot microbubbles (MB) combined with urokinase (UK) mediated ultrasound (US) thrombolysis. Methods: Fifty clots prepared by bovine whole blood were equally divided into 5 groups and were treated in a circulation system with collateral circulation tube. The clots were treated by US, MB and UK in group 1, by US and MB in group 2, by US and UK in group 3 and by UK in group 4. The group 5 was the control group without any treatment. The thrombolysis rate of each group was measured and compared. Residual clots were histologically observed with hematoxylin-eosin staining and immunofluorescence. Results: The thrombolysis rate of group 1 ([73.64±14.16]%) was significantly higher than group 2 ([47.97±11.66]%), group 3 ([57.33±8.65]%), group 4 ([50.85±9.63]%), and group 5 ([29.76±18.06]%, all P<0.05). Histological examination of the clots in group 1 showed multiple thrombolysis foci with clots collapse, and the degradation of fibrin network was further confirmed by immunofluorescence. Conclusion: The intraclot MB mediated US thrombolysis combined with UK can enhance the rate of thrombolysis in vitro experiment.

Original language	English (US)
Pages (from-to)	242-246
Number of pages	5
Journal	Chinese Journal of Interventional Imaging and Therapy
Volume	14
Issue number	4
DOIs	https://doi.org/10.13929/j.1672-8475.201611037
State	Published - Apr 10 2017

Keywords

Microbubbles
Thrombolysis
Thrombosis
Ultrasonics

ASJC Scopus subject areas

Radiology Nuclear Medicine and imaging

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@article{a0e717b34a854be1916892e1f176fd6f,

title = "Intraclot microbubble combined with urokinase mediated ultrasound thrombolysis: Experiment in vitro",

abstract = "Objective: To investigate the efficiency of intraclot microbubbles (MB) combined with urokinase (UK) mediated ultrasound (US) thrombolysis. Methods: Fifty clots prepared by bovine whole blood were equally divided into 5 groups and were treated in a circulation system with collateral circulation tube. The clots were treated by US, MB and UK in group 1, by US and MB in group 2, by US and UK in group 3 and by UK in group 4. The group 5 was the control group without any treatment. The thrombolysis rate of each group was measured and compared. Residual clots were histologically observed with hematoxylin-eosin staining and immunofluorescence. Results: The thrombolysis rate of group 1 ([73.64±14.16]%) was significantly higher than group 2 ([47.97±11.66]%), group 3 ([57.33±8.65]%), group 4 ([50.85±9.63]%), and group 5 ([29.76±18.06]%, all P<0.05). Histological examination of the clots in group 1 showed multiple thrombolysis foci with clots collapse, and the degradation of fibrin network was further confirmed by immunofluorescence. Conclusion: The intraclot MB mediated US thrombolysis combined with UK can enhance the rate of thrombolysis in vitro experiment.",

keywords = "Microbubbles, Thrombolysis, Thrombosis, Ultrasonics",

author = "Qiong Zhu and Shunji Gao and Mengjiao Guo and Yuan Gao and Zheng Liu and Feng Xie",

year = "2017",

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doi = "10.13929/j.1672-8475.201611037",

language = "English (US)",

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T1 - Intraclot microbubble combined with urokinase mediated ultrasound thrombolysis

T2 - Experiment in vitro

AU - Zhu, Qiong

AU - Gao, Shunji

AU - Guo, Mengjiao

AU - Gao, Yuan

AU - Liu, Zheng

AU - Xie, Feng

PY - 2017/4/10

Y1 - 2017/4/10

N2 - Objective: To investigate the efficiency of intraclot microbubbles (MB) combined with urokinase (UK) mediated ultrasound (US) thrombolysis. Methods: Fifty clots prepared by bovine whole blood were equally divided into 5 groups and were treated in a circulation system with collateral circulation tube. The clots were treated by US, MB and UK in group 1, by US and MB in group 2, by US and UK in group 3 and by UK in group 4. The group 5 was the control group without any treatment. The thrombolysis rate of each group was measured and compared. Residual clots were histologically observed with hematoxylin-eosin staining and immunofluorescence. Results: The thrombolysis rate of group 1 ([73.64±14.16]%) was significantly higher than group 2 ([47.97±11.66]%), group 3 ([57.33±8.65]%), group 4 ([50.85±9.63]%), and group 5 ([29.76±18.06]%, all P<0.05). Histological examination of the clots in group 1 showed multiple thrombolysis foci with clots collapse, and the degradation of fibrin network was further confirmed by immunofluorescence. Conclusion: The intraclot MB mediated US thrombolysis combined with UK can enhance the rate of thrombolysis in vitro experiment.

AB - Objective: To investigate the efficiency of intraclot microbubbles (MB) combined with urokinase (UK) mediated ultrasound (US) thrombolysis. Methods: Fifty clots prepared by bovine whole blood were equally divided into 5 groups and were treated in a circulation system with collateral circulation tube. The clots were treated by US, MB and UK in group 1, by US and MB in group 2, by US and UK in group 3 and by UK in group 4. The group 5 was the control group without any treatment. The thrombolysis rate of each group was measured and compared. Residual clots were histologically observed with hematoxylin-eosin staining and immunofluorescence. Results: The thrombolysis rate of group 1 ([73.64±14.16]%) was significantly higher than group 2 ([47.97±11.66]%), group 3 ([57.33±8.65]%), group 4 ([50.85±9.63]%), and group 5 ([29.76±18.06]%, all P<0.05). Histological examination of the clots in group 1 showed multiple thrombolysis foci with clots collapse, and the degradation of fibrin network was further confirmed by immunofluorescence. Conclusion: The intraclot MB mediated US thrombolysis combined with UK can enhance the rate of thrombolysis in vitro experiment.

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