Introduction of cationic charge into DNA near the major groove edge of a guanine•cytosine base pair: Characterization of oligodeoxynucleotides substituted with 7-aminomethyl-7-deaza-2′-deoxyguanosine

(Figure Presented) The replacement of the 7-N atom on guanine (G) with a C-H to give 7-deazaguanine (c⁷G) alters the electronic properties of the heterocyclic base and eliminates a potential major groove cation binding site, which affects the organization of salts and water in the major groove. This has a destabilizing effect on DNA. We report herein the characterization of DNA oligomers containing 7-(aminomethyl)-7-deazaguanine (1) residues using a variety of spectroscopic and thermodynamic approaches. 1 is an intramolecular model for the major groove binding of cations and basic amino acid residues to G. In contrast to c7G, the tethering of a cation in the major groove using 1 affords DNA that is as, or more, stable than the corresponding unmodified DNA. The stabilization is associated with the folding enthalpy and hydration.