TY - GEN
T1 - Investigating sources of fecal contamination in storm drain outfalls
T2 - Water Environment Federation Technical Exhibition and Conference 2017, WEFTEC 2017
AU - Baral, Darshan
AU - Li, Xu
AU - Admiraal, David
AU - Dvorak, Bruce
PY - 2017
Y1 - 2017
N2 - This study investigated the sources of stormwater fecal contamination in Antelope Creek watershed (Lincoln, NE). Stormwater samples from eight different storm drain outfalls in three events and potential source samples (street sweepings, erodible watershed soil, sanitary sewage, and feces of pigeon, swallow, duck, small mammals, dog, and horse) were collected for analysis. The V4 region of the 16S rRNA gene in the DNA extracted from each sample was sequenced and analyzed to identify microbial communities. SourceTracker analysis, carried out based on microbial community, attributed street sweepings as the largest contributor of the fecal indicator population (Bacteroidetes phylum) in stormwater. DNA extracted from the water samples and sanitary sewage were analyzed using the HF183/BacR287 qPCR assay to quantify human fecal contamination. Both SourceTracker analysis and HF183/BacR287 assay revealed sanitary sewage as a minor contributor of contamination in stormwater suggesting there may be sewage exfiltration and/or cross connection in the study area.
AB - This study investigated the sources of stormwater fecal contamination in Antelope Creek watershed (Lincoln, NE). Stormwater samples from eight different storm drain outfalls in three events and potential source samples (street sweepings, erodible watershed soil, sanitary sewage, and feces of pigeon, swallow, duck, small mammals, dog, and horse) were collected for analysis. The V4 region of the 16S rRNA gene in the DNA extracted from each sample was sequenced and analyzed to identify microbial communities. SourceTracker analysis, carried out based on microbial community, attributed street sweepings as the largest contributor of the fecal indicator population (Bacteroidetes phylum) in stormwater. DNA extracted from the water samples and sanitary sewage were analyzed using the HF183/BacR287 qPCR assay to quantify human fecal contamination. Both SourceTracker analysis and HF183/BacR287 assay revealed sanitary sewage as a minor contributor of contamination in stormwater suggesting there may be sewage exfiltration and/or cross connection in the study area.
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M3 - Conference contribution
AN - SCOPUS:85052053992
SN - 9781510863682
T3 - Water Environment Federation Technical Exhibition and Conference 2017, WEFTEC 2017
SP - 3022
EP - 3034
BT - Water Environment Federation Technical Exhibition and Conference 2017, WEFTEC 2017
PB - Water Environment Federation
Y2 - 30 September 2017 through 4 October 2017
ER -