Investigating sources of fecal contamination in storm drain outfalls: Application of genotypic microbial source tracking

Darshan Baral, Xu Li, David Admiraal, Bruce Dvorak

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This study investigated the sources of stormwater fecal contamination in Antelope Creek watershed (Lincoln, NE). Stormwater samples from eight different storm drain outfalls in three events and potential source samples (street sweepings, erodible watershed soil, sanitary sewage, and feces of pigeon, swallow, duck, small mammals, dog, and horse) were collected for analysis. The V4 region of the 16S rRNA gene in the DNA extracted from each sample was sequenced and analyzed to identify microbial communities. SourceTracker analysis, carried out based on microbial community, attributed street sweepings as the largest contributor of the fecal indicator population (Bacteroidetes phylum) in stormwater. DNA extracted from the water samples and sanitary sewage were analyzed using the HF183/BacR287 qPCR assay to quantify human fecal contamination. Both SourceTracker analysis and HF183/BacR287 assay revealed sanitary sewage as a minor contributor of contamination in stormwater suggesting there may be sewage exfiltration and/or cross connection in the study area.

Original languageEnglish (US)
Title of host publicationWater Environment Federation Technical Exhibition and Conference 2017, WEFTEC 2017
PublisherWater Environment Federation
Pages3022-3034
Number of pages13
ISBN (Print)9781510863682
StatePublished - Jan 1 2017
EventWater Environment Federation Technical Exhibition and Conference 2017, WEFTEC 2017 - Chicago, United States
Duration: Sep 30 2017Oct 4 2017

Publication series

NameWater Environment Federation Technical Exhibition and Conference 2017, WEFTEC 2017
Volume5

Other

OtherWater Environment Federation Technical Exhibition and Conference 2017, WEFTEC 2017
CountryUnited States
CityChicago
Period9/30/1710/4/17

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ASJC Scopus subject areas

  • Pollution
  • Ecological Modeling
  • Environmental Chemistry
  • Waste Management and Disposal
  • Water Science and Technology

Cite this

Baral, D., Li, X., Admiraal, D., & Dvorak, B. (2017). Investigating sources of fecal contamination in storm drain outfalls: Application of genotypic microbial source tracking. In Water Environment Federation Technical Exhibition and Conference 2017, WEFTEC 2017 (pp. 3022-3034). (Water Environment Federation Technical Exhibition and Conference 2017, WEFTEC 2017; Vol. 5). Water Environment Federation.