Investigating the human immunodeficiency virus type 1-infected monocyte-derived macrophage secretome

Pawel Ciborowski, Irena Kadiu, Wojciech Rozek, Lynette Smith, Kristen Bernhardt, Melissa Fladseth, Mary Ricardo-Dukelow, Howard E. Gendelman

Research output: Contribution to journalArticle

70 Scopus citations

Abstract

Mononuclear phagocytes (bone marrow monocyte-derived macrophages, alveolar macrophages, perivascular macrophages, and microglia) are reservoirs and vehicles of dissemination for the human immunodeficiency virus type-1 (HIV-1). How virus alters mononuclear phagocyte immunoregulatory activities to complete its life cycle and influence disease is incompletely understood. In attempts to better understanding the influence of virus on macrophage functions, we used one-dimensional electrophoresis, and liquid chromatography tandem mass spectrometry to analyze the secretome of HIV-1-infected human monocyte-derived macrophages. We identified 110 proteins in culture supernatants of control (uninfected) and virus-infected cells. Differentially expressed cytoskeletal, enzymes, redox, and immunoregulatory protein classes were discovered and validated by Western blot tests. These included, but were not limited to, cystatin C, cystatin B, chitinase 3-like 1 protein, cofilin-1, l-plastin, superoxide dismutase, leukotriene A4 hydrolase, and α-enolase. This study, using a unique proteomics platform, provides novel insights into virus-host cell interactions that likely affect the functional role of macrophages in HIV disease.

Original languageEnglish (US)
Pages (from-to)198-209
Number of pages12
JournalVirology
Volume363
Issue number1
DOIs
StatePublished - Jun 20 2007

Keywords

  • HIV-1-associated dementia
  • Human immunodeficiency virus
  • Monocyte-derived macrophages
  • Proteomics
  • Secretome

ASJC Scopus subject areas

  • Virology

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