TY - JOUR
T1 - Investigating the human immunodeficiency virus type 1-infected monocyte-derived macrophage secretome
AU - Ciborowski, Pawel
AU - Kadiu, Irena
AU - Rozek, Wojciech
AU - Smith, Lynette
AU - Bernhardt, Kristen
AU - Fladseth, Melissa
AU - Ricardo-Dukelow, Mary
AU - Gendelman, Howard E.
N1 - Funding Information:
The work was supported by NIH Grants 5 R37 NS36126-07, 5 R01 NS034239-10, 1 P01 NS43985-01A1, 5 P01NS31492-11, 5 R01 MH64570-03, P01 NS11766-28 (to H.E.G.); 20 RR15635 from the COBRE Program of the National Center for Research Resources (to P.C. and H.E.G.); and 1R21 MH075662-01 (to P.C.). The authors are grateful to Ms. Robin Taylor for outstanding administrative and computer support.
PY - 2007/6/20
Y1 - 2007/6/20
N2 - Mononuclear phagocytes (bone marrow monocyte-derived macrophages, alveolar macrophages, perivascular macrophages, and microglia) are reservoirs and vehicles of dissemination for the human immunodeficiency virus type-1 (HIV-1). How virus alters mononuclear phagocyte immunoregulatory activities to complete its life cycle and influence disease is incompletely understood. In attempts to better understanding the influence of virus on macrophage functions, we used one-dimensional electrophoresis, and liquid chromatography tandem mass spectrometry to analyze the secretome of HIV-1-infected human monocyte-derived macrophages. We identified 110 proteins in culture supernatants of control (uninfected) and virus-infected cells. Differentially expressed cytoskeletal, enzymes, redox, and immunoregulatory protein classes were discovered and validated by Western blot tests. These included, but were not limited to, cystatin C, cystatin B, chitinase 3-like 1 protein, cofilin-1, l-plastin, superoxide dismutase, leukotriene A4 hydrolase, and α-enolase. This study, using a unique proteomics platform, provides novel insights into virus-host cell interactions that likely affect the functional role of macrophages in HIV disease.
AB - Mononuclear phagocytes (bone marrow monocyte-derived macrophages, alveolar macrophages, perivascular macrophages, and microglia) are reservoirs and vehicles of dissemination for the human immunodeficiency virus type-1 (HIV-1). How virus alters mononuclear phagocyte immunoregulatory activities to complete its life cycle and influence disease is incompletely understood. In attempts to better understanding the influence of virus on macrophage functions, we used one-dimensional electrophoresis, and liquid chromatography tandem mass spectrometry to analyze the secretome of HIV-1-infected human monocyte-derived macrophages. We identified 110 proteins in culture supernatants of control (uninfected) and virus-infected cells. Differentially expressed cytoskeletal, enzymes, redox, and immunoregulatory protein classes were discovered and validated by Western blot tests. These included, but were not limited to, cystatin C, cystatin B, chitinase 3-like 1 protein, cofilin-1, l-plastin, superoxide dismutase, leukotriene A4 hydrolase, and α-enolase. This study, using a unique proteomics platform, provides novel insights into virus-host cell interactions that likely affect the functional role of macrophages in HIV disease.
KW - HIV-1-associated dementia
KW - Human immunodeficiency virus
KW - Monocyte-derived macrophages
KW - Proteomics
KW - Secretome
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U2 - 10.1016/j.virol.2007.01.013
DO - 10.1016/j.virol.2007.01.013
M3 - Article
C2 - 17320137
AN - SCOPUS:34247553696
SN - 0042-6822
VL - 363
SP - 198
EP - 209
JO - Virology
JF - Virology
IS - 1
ER -