Investigation of contamination sources in a small urban creek using microbial source tracking and isotopic analysis

Darshan Baral, Allison Speicher, Bruce I. Dvorak, David M. Admiraal, Xu Li

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

High concentrations of E. coli and nutrients have been detected in Antelope Creek, a small urban stream, in Lincoln. The objective of this study is to investigate the sources of microbes and nutrients in the watershed using microbial source tracking, nutrient isotopic analyses, and traditional water quality analyses (nutrients, E. coli quantification, etc.). Wet and dry weather water, scat, vegetation, soil, and stream-bed sediment samples were collected over a two year period (June 2013-October 2014). The scat samples were from species observed in the vicinity of the stream channel. DNA extracts from the collected samples were amplified using a PCR primer set targeting the V4 region of the 16S rRNA Gene. Sequence libraries were established using the Illumina sequencing technology. Isotopic analysis of nitrate, oxygen, and phosphate in the water, soil, and vegetation samples was also performed. Preliminary analysis using source tracking shows distinct microbial compositions among wet weather water, dry weather water, and stream-bed sediment samples. Results from nutrient and preliminary isotopic analysis suggest that atmospheric deposition may be an important contributor of inorganic nitrogen.

Original languageEnglish (US)
Title of host publicationWorld Environmental and Water Resources Congress 2015
Subtitle of host publicationFloods, Droughts, and Ecosystems - Proceedings of the 2015 World Environmental and Water Resources Congress
EditorsVeronica L. Webster, Karen Karvazy
PublisherAmerican Society of Civil Engineers (ASCE)
Pages421-430
Number of pages10
ISBN (Electronic)9780784479162
DOIs
StatePublished - 2015
EventWorld Environmental and Water Resources Congress 2015: Floods, Droughts, and Ecosystems - Austin, United States
Duration: May 17 2015May 21 2015

Publication series

NameWorld Environmental and Water Resources Congress 2015: Floods, Droughts, and Ecosystems - Proceedings of the 2015 World Environmental and Water Resources Congress

Other

OtherWorld Environmental and Water Resources Congress 2015: Floods, Droughts, and Ecosystems
CountryUnited States
CityAustin
Period5/17/155/21/15

ASJC Scopus subject areas

  • Water Science and Technology

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    Baral, D., Speicher, A., Dvorak, B. I., Admiraal, D. M., & Li, X. (2015). Investigation of contamination sources in a small urban creek using microbial source tracking and isotopic analysis. In V. L. Webster, & K. Karvazy (Eds.), World Environmental and Water Resources Congress 2015: Floods, Droughts, and Ecosystems - Proceedings of the 2015 World Environmental and Water Resources Congress (pp. 421-430). (World Environmental and Water Resources Congress 2015: Floods, Droughts, and Ecosystems - Proceedings of the 2015 World Environmental and Water Resources Congress). American Society of Civil Engineers (ASCE). https://doi.org/10.1061/9780784479162.038