TY - GEN
T1 - Investigations of bio markers for human lymphoblastoid cells using atomic force microscopy
AU - Yang, Ruiguo
AU - Xi, Ning
AU - Fung, Carmen Kar Man
AU - Lai, King Wai Chiu
AU - Seiffert-Sinha, Kristina
AU - Sinha, Animesh A.
AU - Zhang, Weijing
PY - 2010
Y1 - 2010
N2 - We report the use of Atomic Force Microcopy (AFM) in combination with Fluorescence Microscopy (FM) for the investigation of non-Hodgkin's type B cells lymphoma after antibody treatment. Rituximab is used in the treatment this cancer type. It is a chimeric monoclonal antibody directed against the protein Cluster of Differentiation 20 (CD20), which is expressed on mature B cells, including the B cell lymphoma surface. We applied anti-CD20 antibody to the B lymphoma cells that was labeled with fluorophore. Simultaneous imaging was then performed by both FM and AFM. Immunofluorescence imaging confirms the binding of Rituximab to the CD20 protein; while the AFM imaging revealed changes in lymphoma cells in terms of mean cell height and cell surface roughness. In addition, our AFM based nanomanipulation system can assist in the analysis of mechanical property of the cell by recording the force-displacement curves at the cell surface. AFM imaging and measurements may provide biomarkers of cell behavior and may facilitate the treatment of lymphoma clinically as a pretest to determine the effectiveness of the drug Rituximab on lymphoma cells from individual patients.
AB - We report the use of Atomic Force Microcopy (AFM) in combination with Fluorescence Microscopy (FM) for the investigation of non-Hodgkin's type B cells lymphoma after antibody treatment. Rituximab is used in the treatment this cancer type. It is a chimeric monoclonal antibody directed against the protein Cluster of Differentiation 20 (CD20), which is expressed on mature B cells, including the B cell lymphoma surface. We applied anti-CD20 antibody to the B lymphoma cells that was labeled with fluorophore. Simultaneous imaging was then performed by both FM and AFM. Immunofluorescence imaging confirms the binding of Rituximab to the CD20 protein; while the AFM imaging revealed changes in lymphoma cells in terms of mean cell height and cell surface roughness. In addition, our AFM based nanomanipulation system can assist in the analysis of mechanical property of the cell by recording the force-displacement curves at the cell surface. AFM imaging and measurements may provide biomarkers of cell behavior and may facilitate the treatment of lymphoma clinically as a pretest to determine the effectiveness of the drug Rituximab on lymphoma cells from individual patients.
KW - AFM live cell imaging
KW - Atomic force microscopy
KW - Cell immobilization
KW - Fluorescence microscopy
KW - Human lymphoblastoid cells
KW - Rituximab
UR - http://www.scopus.com/inward/record.url?scp=78649277683&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=78649277683&partnerID=8YFLogxK
U2 - 10.1109/NEMS.2010.5592162
DO - 10.1109/NEMS.2010.5592162
M3 - Conference contribution
AN - SCOPUS:78649277683
SN - 9781424465439
T3 - 2010 IEEE 5th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010
SP - 129
EP - 132
BT - 2010 IEEE 5th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010
T2 - 5th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010
Y2 - 20 January 2010 through 23 January 2010
ER -