Involvement of both PKS and NRPS in antibacterial activity in Lysobacter enzymogenes OH11

Juan Zhang, Liangcheng Du, Fengquan Liu, Feifei Xu, Baishi Hu, Vittorio Venturi, Guoliang Qian

Research output: Contribution to journalArticlepeer-review

20 Scopus citations


Polyketides and nonribosomal peptides represent two large families of natural products (NPs) with diverse structures and important functions. They are synthesized by polyketide synthase (PKS) and nonribosomal peptide synthetase (NRPS), respectively. Lysobacter enzymogenes is emerging as a novel biocontrol agent against pathogens of crop plants and a new source of bioactive NPs, such as antibacterial antibiotic WAP-8294A2 and antifungal antibiotic HSAF. Genome survey of strain OH11, a Chinese L. enzymogenes isolate, detected four novel PKS, NRPS or hybrid gene clusters, designed as cluster A to D. We further individually mutated five genes (PKS or NRPS) located in these four gene clusters and showed that a PKS gene in cluster A and an NRPS gene in cluster D were involved in the antibacterial activity via a WAP-8294A2 dependent way. The data also showed that none of the five genes was associated with antifungal activity and the regulation of HSAF biosynthesis. Our results reveal the unusual regulatory role of these PKS and NRPS genes that were discovered from genome mining in L. enzymogenes.

Original languageEnglish (US)
Pages (from-to)170-176
Number of pages7
JournalFEMS Microbiology Letters
Issue number2
StatePublished - Jun 2014


  • Lysobacter enzymogenes
  • Nonribosomal peptide synthetase
  • Polyketide synthase
  • WAP-8294A2

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics


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