TY - JOUR
T1 - Involvement of both PKS and NRPS in antibacterial activity in Lysobacter enzymogenes OH11
AU - Zhang, Juan
AU - Du, Liangcheng
AU - Liu, Fengquan
AU - Xu, Feifei
AU - Hu, Baishi
AU - Venturi, Vittorio
AU - Qian, Guoliang
PY - 2014/6
Y1 - 2014/6
N2 - Polyketides and nonribosomal peptides represent two large families of natural products (NPs) with diverse structures and important functions. They are synthesized by polyketide synthase (PKS) and nonribosomal peptide synthetase (NRPS), respectively. Lysobacter enzymogenes is emerging as a novel biocontrol agent against pathogens of crop plants and a new source of bioactive NPs, such as antibacterial antibiotic WAP-8294A2 and antifungal antibiotic HSAF. Genome survey of strain OH11, a Chinese L. enzymogenes isolate, detected four novel PKS, NRPS or hybrid gene clusters, designed as cluster A to D. We further individually mutated five genes (PKS or NRPS) located in these four gene clusters and showed that a PKS gene in cluster A and an NRPS gene in cluster D were involved in the antibacterial activity via a WAP-8294A2 dependent way. The data also showed that none of the five genes was associated with antifungal activity and the regulation of HSAF biosynthesis. Our results reveal the unusual regulatory role of these PKS and NRPS genes that were discovered from genome mining in L. enzymogenes.
AB - Polyketides and nonribosomal peptides represent two large families of natural products (NPs) with diverse structures and important functions. They are synthesized by polyketide synthase (PKS) and nonribosomal peptide synthetase (NRPS), respectively. Lysobacter enzymogenes is emerging as a novel biocontrol agent against pathogens of crop plants and a new source of bioactive NPs, such as antibacterial antibiotic WAP-8294A2 and antifungal antibiotic HSAF. Genome survey of strain OH11, a Chinese L. enzymogenes isolate, detected four novel PKS, NRPS or hybrid gene clusters, designed as cluster A to D. We further individually mutated five genes (PKS or NRPS) located in these four gene clusters and showed that a PKS gene in cluster A and an NRPS gene in cluster D were involved in the antibacterial activity via a WAP-8294A2 dependent way. The data also showed that none of the five genes was associated with antifungal activity and the regulation of HSAF biosynthesis. Our results reveal the unusual regulatory role of these PKS and NRPS genes that were discovered from genome mining in L. enzymogenes.
KW - Lysobacter enzymogenes
KW - Nonribosomal peptide synthetase
KW - Polyketide synthase
KW - WAP-8294A2
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U2 - 10.1111/1574-6968.12457
DO - 10.1111/1574-6968.12457
M3 - Article
C2 - 24801439
AN - SCOPUS:84902532964
SN - 0378-1097
VL - 355
SP - 170
EP - 176
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
IS - 2
ER -