Ionic liquids as solvents for in situ dispersive liquid-liquid microextraction of DNA

Tianhao Li; Manishkumar D. Joshi; Donald R. Ronning; Jared L. Anderson

doi:10.1016/j.chroma.2012.11.055

Ionic liquids as solvents for in situ dispersive liquid-liquid microextraction of DNA

Tianhao Li, Manishkumar D. Joshi, Donald R. Ronning, Jared L. Anderson

Research output: Contribution to journal › Article › peer-review

70 Scopus citations

Abstract

Six ionic liquids (ILs) were applied for the first time as solvents in the extraction and preconcentration of deoxyribonucleic acid (DNA) using an in situ dispersive liquid-liquid microextraction (DLLME) approach. The effect of different IL substituents and functional group on the extraction efficiency of DNA was investigated. The highest extraction efficiencies of DNA were obtained using 1-(1,2-dihydroxypropyl)-3-hexadecylimidazolium bromide (C₁₆POHIM-Br) and N,N-didecyl-N-methyl-d-glucaminium bromide [(C₁₀)₂NMDG-Br]. Extraction efficiencies higher than 97% were obtained using small amounts of IL (0.50mg) for each extraction. The extraction of DNA from a sample matrix containing metal ions and protein revealed that the metal ions did not interfere with the extraction of DNA and that the co-extraction of protein can be mitigated by performing the extraction under moderately acidic conditions. Data from ³¹P NMR spectroscopy suggest that a combination of electrostatic and π-π interactions dominate IL-DNA complexation and that the extraction is concentration dependant.

Original language	English (US)
Pages (from-to)	8-14
Number of pages	7
Journal	Journal of Chromatography A
Volume	1272
DOIs	https://doi.org/10.1016/j.chroma.2012.11.055
State	Published - Jan 11 2013
Externally published	Yes

Keywords

DNA extraction
Dispersive liquid-liquid microextraction
Ionic liquid
Nucleic acid

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Organic Chemistry

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10.1016/j.chroma.2012.11.055

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title = "Ionic liquids as solvents for in situ dispersive liquid-liquid microextraction of DNA",

abstract = "Six ionic liquids (ILs) were applied for the first time as solvents in the extraction and preconcentration of deoxyribonucleic acid (DNA) using an in situ dispersive liquid-liquid microextraction (DLLME) approach. The effect of different IL substituents and functional group on the extraction efficiency of DNA was investigated. The highest extraction efficiencies of DNA were obtained using 1-(1,2-dihydroxypropyl)-3-hexadecylimidazolium bromide (C16POHIM-Br) and N,N-didecyl-N-methyl-d-glucaminium bromide [(C10)2NMDG-Br]. Extraction efficiencies higher than 97% were obtained using small amounts of IL (0.50mg) for each extraction. The extraction of DNA from a sample matrix containing metal ions and protein revealed that the metal ions did not interfere with the extraction of DNA and that the co-extraction of protein can be mitigated by performing the extraction under moderately acidic conditions. Data from 31P NMR spectroscopy suggest that a combination of electrostatic and π-π interactions dominate IL-DNA complexation and that the extraction is concentration dependant.",

keywords = "DNA extraction, Dispersive liquid-liquid microextraction, Ionic liquid, Nucleic acid",

author = "Tianhao Li and Joshi, {Manishkumar D.} and Ronning, {Donald R.} and Anderson, {Jared L.}",

note = "Funding Information: J. L. A. acknowledges funding from the Analytical and Surface Chemistry Program in the Division of Chemistry and the Separation and Purification Processes Program in the Chemical, Environmental, Bioengineering, and Transport Systems Division from the National Science Foundation for a CAREER grant ( CHE-0748612 ). ",

year = "2013",

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doi = "10.1016/j.chroma.2012.11.055",

language = "English (US)",

volume = "1272",

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journal = "Journal of Chromatography A",

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T1 - Ionic liquids as solvents for in situ dispersive liquid-liquid microextraction of DNA

AU - Li, Tianhao

AU - Joshi, Manishkumar D.

AU - Ronning, Donald R.

AU - Anderson, Jared L.

N1 - Funding Information: J. L. A. acknowledges funding from the Analytical and Surface Chemistry Program in the Division of Chemistry and the Separation and Purification Processes Program in the Chemical, Environmental, Bioengineering, and Transport Systems Division from the National Science Foundation for a CAREER grant ( CHE-0748612 ).

PY - 2013/1/11

Y1 - 2013/1/11

N2 - Six ionic liquids (ILs) were applied for the first time as solvents in the extraction and preconcentration of deoxyribonucleic acid (DNA) using an in situ dispersive liquid-liquid microextraction (DLLME) approach. The effect of different IL substituents and functional group on the extraction efficiency of DNA was investigated. The highest extraction efficiencies of DNA were obtained using 1-(1,2-dihydroxypropyl)-3-hexadecylimidazolium bromide (C16POHIM-Br) and N,N-didecyl-N-methyl-d-glucaminium bromide [(C10)2NMDG-Br]. Extraction efficiencies higher than 97% were obtained using small amounts of IL (0.50mg) for each extraction. The extraction of DNA from a sample matrix containing metal ions and protein revealed that the metal ions did not interfere with the extraction of DNA and that the co-extraction of protein can be mitigated by performing the extraction under moderately acidic conditions. Data from 31P NMR spectroscopy suggest that a combination of electrostatic and π-π interactions dominate IL-DNA complexation and that the extraction is concentration dependant.

AB - Six ionic liquids (ILs) were applied for the first time as solvents in the extraction and preconcentration of deoxyribonucleic acid (DNA) using an in situ dispersive liquid-liquid microextraction (DLLME) approach. The effect of different IL substituents and functional group on the extraction efficiency of DNA was investigated. The highest extraction efficiencies of DNA were obtained using 1-(1,2-dihydroxypropyl)-3-hexadecylimidazolium bromide (C16POHIM-Br) and N,N-didecyl-N-methyl-d-glucaminium bromide [(C10)2NMDG-Br]. Extraction efficiencies higher than 97% were obtained using small amounts of IL (0.50mg) for each extraction. The extraction of DNA from a sample matrix containing metal ions and protein revealed that the metal ions did not interfere with the extraction of DNA and that the co-extraction of protein can be mitigated by performing the extraction under moderately acidic conditions. Data from 31P NMR spectroscopy suggest that a combination of electrostatic and π-π interactions dominate IL-DNA complexation and that the extraction is concentration dependant.

KW - DNA extraction

KW - Dispersive liquid-liquid microextraction

KW - Ionic liquid

KW - Nucleic acid

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Ionic liquids as solvents for in situ dispersive liquid-liquid microextraction of DNA

Abstract

Keywords

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