Ionic liquids as solvents for in situ dispersive liquid-liquid microextraction of DNA

Tianhao Li, Manishkumar D. Joshi, Donald R. Ronning, Jared L. Anderson

Research output: Contribution to journalArticle

55 Scopus citations

Abstract

Six ionic liquids (ILs) were applied for the first time as solvents in the extraction and preconcentration of deoxyribonucleic acid (DNA) using an in situ dispersive liquid-liquid microextraction (DLLME) approach. The effect of different IL substituents and functional group on the extraction efficiency of DNA was investigated. The highest extraction efficiencies of DNA were obtained using 1-(1,2-dihydroxypropyl)-3-hexadecylimidazolium bromide (C16POHIM-Br) and N,N-didecyl-N-methyl-d-glucaminium bromide [(C10)2NMDG-Br]. Extraction efficiencies higher than 97% were obtained using small amounts of IL (0.50mg) for each extraction. The extraction of DNA from a sample matrix containing metal ions and protein revealed that the metal ions did not interfere with the extraction of DNA and that the co-extraction of protein can be mitigated by performing the extraction under moderately acidic conditions. Data from 31P NMR spectroscopy suggest that a combination of electrostatic and π-π interactions dominate IL-DNA complexation and that the extraction is concentration dependant.

Original languageEnglish (US)
Pages (from-to)8-14
Number of pages7
JournalJournal of Chromatography A
Volume1272
DOIs
StatePublished - Jan 11 2013

Keywords

  • DNA extraction
  • Dispersive liquid-liquid microextraction
  • Ionic liquid
  • Nucleic acid

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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