IPSC-derived fibroblasts demonstrate augmented production and assembly of extracellular matrix proteins

Yulia Shamis, Kyle J. Hewitt, Susan E. Bear, Alt Holland Addy, Hiba Qari, Mariam Margvelashvilli, Elana B. Knight, Avi Smith, Jonathan A. Garlick

Research output: Contribution to journalArticlepeer-review

25 Scopus citations


Reprogramming of somatic cells to induced pluripotent stem cells (iPSC) provides an important cell source to derive patient-specific cells for potential therapeutic applications. However, it is not yet clear whether reprogramming through pluripotency allows the production of differentiated cells with improved functional properties that may be beneficial in regenerative therapies. To address this, we compared the production and assembly of extracellular matrix (ECM) by iPSC-derived fibroblasts to that of the parental, dermal fibroblasts (BJ), from which these iPSC were initially reprogrammed, and to fibroblasts differentiated from human embryonic stem cells (hESC). iPSC-and hESC-derived fibroblasts demonstrated stable expressionof surface markers characteristic of stromal fibroblasts during prolonged culture and showed an elevated growth potential when compared to the parental BJ fibroblasts. We found that in the presence of L-ascorbic acid-2-phosphate, iPSC-and hESC-derived fibroblasts increased their expression of collagen genes, secretion of soluble collagen, and extracellular deposition of type I collagen to a significantly greater degree than that seen in the parental BJ fibroblasts. Under culture conditions that enabled the self-assembly of a 3D stromal tissue, iPSC-and hESC-derived fibroblasts generated a well organized, ECM that was enriched in type III collagen. By characterizing the functional properties of iPSC-derived fibroblasts compared to their parental fibroblasts, we demonstrate that these cells represent a promising, alternative source of fibroblasts to advance future regenerative therapies.

Original languageEnglish (US)
Pages (from-to)112-122
Number of pages11
JournalIn Vitro Cellular and Developmental Biology - Animal
Issue number2
StatePublished - Feb 2012
Externally publishedYes


  • Ascorbic acid
  • Extracellular matrix
  • Fibroblasts
  • Human embryonic stem cells
  • Induced pluripotent stem cells

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology


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