IRF3 and ERK MAP-kinases control nitric oxide production from macrophages in response to poly-I:C

Tyler C. Moore, Thomas M. Petro

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Understanding nitric oxide (NO) in innate anti-viral immunity and immune-mediated pathology is hampered by incomplete details of its transcriptional and signaling factors. We found in macrophages that IRF3, ERK MAP-kinases, and PKR are essential to NO production in response to RNA-virus mimic, poly I:C, a TLR3 agonist. ERK's role in NO induction may be through phosphorylation of serine-171 of IRF3 and expression of NO-inducing cytokines, IL-6 and IFN-β. However, these cytokines induced less NO in IRF3 knockout or knockdown macrophages. These findings show that ERK and IRF3 coordinate induction of NO by macrophages in response to stimulation of TLR3.

Original languageEnglish (US)
Pages (from-to)3014-3020
Number of pages7
JournalFEBS Letters
Volume587
Issue number18
DOIs
StatePublished - Sep 17 2013

Keywords

  • ERK MAP-kinase
  • Interferon response factor-3
  • Interleukin-6
  • Macrophages
  • Nitric oxide
  • Poly I:C

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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