Isoenzymes Of ß-Hexosaminidase From Normal Rat Colon And Colonic Carcinoma

Michael G. Brattain, Catherine Green, Pamela M. Kimball, Michael Marks, Mahnaz Khaled

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

N-Acetyl-ß-D-hexosaminidase isolated from normal rat colon was compared to that obtained from a transplantable rat colonic carcinoma. Levels of total hexosaminidase from purified epithelial cells of normal colon were similar to those from purified malignant cells from the transplantable tumor. Cultured malignant cells had significantly higher levels of activity than did freshly purified tumor cells. Isoelectric focusing of hexosaminidase from normal rat colon indicated approximately equal amounts of A (pl 5.0) and B (pl 8.1) isoenzyme activity. The B isoenzyme (normal cell) was more stable to heat inactivation than was the A isoenzyme and had significantly higher activity at low pH's. In contrast, the B isoenzyme from the tumor was relatively unstable to heat and low pH. Downloaded from cancerres.aacrjournals.org on February 9, 2016.

Original languageEnglish (US)
Pages (from-to)4083-4090
Number of pages8
JournalCancer Research
Volume39
Issue number10
StatePublished - Oct 1 1979
Externally publishedYes

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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    Brattain, M. G., Green, C., Kimball, P. M., Marks, M., & Khaled, M. (1979). Isoenzymes Of ß-Hexosaminidase From Normal Rat Colon And Colonic Carcinoma. Cancer Research, 39(10), 4083-4090.