Isolation and characterization of a cDNA encoding maize cytosolic malate dehydrogenase

Jian Guan Hu; Xiang Shan Zhao; Jun Liu; Zi Qiang Yuan; Jin Shui Yang

Isolation and characterization of a cDNA encoding maize cytosolic malate dehydrogenase

Jian Guan Hu, Xiang Shan Zhao, Jun Liu, Zi Qiang Yuan, Jin Shui Yang

Research output: Contribution to journal › Article › peer-review

Abstract

A cDNA fragment derived from a gene over-expressing in hybrid maize (Zea mays L.) was isolated with RT-PCR and used as probe to screen cDNA library of hybrid maize seedlings. A positive cDNA clone ZH02 corresponding to the full-length mRNA sequence was obtained, which was shown to have an open reading frame encoding 332 a.a. DNA and proteinase database search revealed that the deduced amino acid sequence of ZH02 has high similarity with the cMDH of Mesembryanthemum crystallium L. and Arabidopsis thaliana (L.) Heynh. up to 90% and 84%, respectively. This is the first report of the full-length gene coding for the cereal cMDH.

Original language	English (US)
Pages (from-to)	40-44
Number of pages	5
Journal	Acta Botanica Sinica
Volume	41
Issue number	1
State	Published - Jan 1999
Externally published	Yes

Keywords

Cytosolic malate dehydrogenase
Gene cloning
Heterosis
Maize

ASJC Scopus subject areas

Biochemistry
Biochemistry, Genetics and Molecular Biology(all)
Plant Science

Cite this

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keywords = "Cytosolic malate dehydrogenase, Gene cloning, Heterosis, Maize",

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AU - Hu, Jian Guan

AU - Zhao, Xiang Shan

AU - Liu, Jun

AU - Yuan, Zi Qiang

AU - Yang, Jin Shui

PY - 1999/1

Y1 - 1999/1

N2 - A cDNA fragment derived from a gene over-expressing in hybrid maize (Zea mays L.) was isolated with RT-PCR and used as probe to screen cDNA library of hybrid maize seedlings. A positive cDNA clone ZH02 corresponding to the full-length mRNA sequence was obtained, which was shown to have an open reading frame encoding 332 a.a. DNA and proteinase database search revealed that the deduced amino acid sequence of ZH02 has high similarity with the cMDH of Mesembryanthemum crystallium L. and Arabidopsis thaliana (L.) Heynh. up to 90% and 84%, respectively. This is the first report of the full-length gene coding for the cereal cMDH.

AB - A cDNA fragment derived from a gene over-expressing in hybrid maize (Zea mays L.) was isolated with RT-PCR and used as probe to screen cDNA library of hybrid maize seedlings. A positive cDNA clone ZH02 corresponding to the full-length mRNA sequence was obtained, which was shown to have an open reading frame encoding 332 a.a. DNA and proteinase database search revealed that the deduced amino acid sequence of ZH02 has high similarity with the cMDH of Mesembryanthemum crystallium L. and Arabidopsis thaliana (L.) Heynh. up to 90% and 84%, respectively. This is the first report of the full-length gene coding for the cereal cMDH.

KW - Cytosolic malate dehydrogenase

KW - Gene cloning

KW - Heterosis

KW - Maize

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Isolation and characterization of a cDNA encoding maize cytosolic malate dehydrogenase

Abstract

Keywords

ASJC Scopus subject areas

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