Isolation and characterization of a cDNA encoding maize cytosolic malate dehydrogenase

Jian Guan Hu, Xiang Shan Zhao, Jun Liu, Zi Qiang Yuan, Jin Shui Yang

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

A cDNA fragment derived from a gene over-expressing in hybrid maize (Zea mays L.) was isolated with RT-PCR and used as probe to screen cDNA library of hybrid maize seedlings. A positive cDNA clone ZH02 corresponding to the full-length mRNA sequence was obtained, which was shown to have an open reading frame encoding 332 a.a. DNA and proteinase database search revealed that the deduced amino acid sequence of ZH02 has high similarity with the cMDH of Mesembryanthemum crystallium L. and Arabidopsis thaliana (L.) Heynh. up to 90% and 84%, respectively. This is the first report of the full-length gene coding for the cereal cMDH.

Original languageEnglish (US)
Pages (from-to)40-44
Number of pages5
JournalActa Botanica Sinica
Volume41
Issue number1
StatePublished - Jan 1999

Keywords

  • Cytosolic malate dehydrogenase
  • Gene cloning
  • Heterosis
  • Maize

ASJC Scopus subject areas

  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Plant Science

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    Hu, J. G., Zhao, X. S., Liu, J., Yuan, Z. Q., & Yang, J. S. (1999). Isolation and characterization of a cDNA encoding maize cytosolic malate dehydrogenase. Acta Botanica Sinica, 41(1), 40-44.