Isolation and characterization of the murine transforming growth factor-β2 promoter

Phillip J. Wilder, Michelle M. Desler, Tamara K. Nowling, Angie Rizzino

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

This report describes the isolation and characterization of the 5′ flanking region of the murine transforming growth factor beta-2 (TGF-β2) gene. A genomic clone containing the promoter region of the gene was isolated after screening a bacteriophage P1 genomic library. The resulting clone was sequenced and compared to promoters for the human and chicken TGF-β2 genes. The sequence located near the transcription start site is highly conserved. It includes a TATA box, an E-box, and a largely conserved CRE/ATF site. A series of murine TGF-β2 promoter/reporter constructs was generated to identify regulatory regions of the gene. As in the case of the human TGF-β2 gene, sequences just upstream of the TATA box, including the CRE/ATF site, actively stimulate the murine TGF-β2 promoter. However, unlike the human TGF-β2 gene, the 5′ flanking region of the murine TGF-β2 gene contains a long alternating purine/pyrimidine repeat that unexpectedly exerts a strong positive effect on its promoter. This is of particular interest since alternating purine/pyrimidine repeats in other promoters have been observed to be inhibitory.

Original languageEnglish (US)
Pages (from-to)201-209
Number of pages9
JournalGene
Volume270
Issue number1-2
DOIs
StatePublished - May 30 2001

Keywords

  • Alternating purine pyrimidine repeat
  • CRE/ATF
  • E-box
  • Embryonal carcinoma cells

ASJC Scopus subject areas

  • Genetics

Fingerprint Dive into the research topics of 'Isolation and characterization of the murine transforming growth factor-β2 promoter'. Together they form a unique fingerprint.

  • Cite this