This report describes the isolation and characterization of the 5′ flanking region of the murine transforming growth factor beta-2 (TGF-β2) gene. A genomic clone containing the promoter region of the gene was isolated after screening a bacteriophage P1 genomic library. The resulting clone was sequenced and compared to promoters for the human and chicken TGF-β2 genes. The sequence located near the transcription start site is highly conserved. It includes a TATA box, an E-box, and a largely conserved CRE/ATF site. A series of murine TGF-β2 promoter/reporter constructs was generated to identify regulatory regions of the gene. As in the case of the human TGF-β2 gene, sequences just upstream of the TATA box, including the CRE/ATF site, actively stimulate the murine TGF-β2 promoter. However, unlike the human TGF-β2 gene, the 5′ flanking region of the murine TGF-β2 gene contains a long alternating purine/pyrimidine repeat that unexpectedly exerts a strong positive effect on its promoter. This is of particular interest since alternating purine/pyrimidine repeats in other promoters have been observed to be inhibitory.
- Alternating purine pyrimidine repeat
- Embryonal carcinoma cells
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