Isolation and cloning of a new gene encoding maize initiation factor

Jianguang Hu; Xiangshan Zhao; Ziqiang Yuan; Xiaoying Qian; Jinshui Yang

doi:10.1007/bf02884536

Isolation and cloning of a new gene encoding maize initiation factor

Jianguang Hu, Xiangshan Zhao, Ziqiang Yuan, Xiaoying Qian, Jinshui Yang

Genetics, Cell Biology & Anatomy

Research output: Contribution to journal › Article › peer-review

Abstract

It was demonstrated that parental gene expression of maize in hybrid genetic background was different from that in their inbred lines. RT-PCR revealed a cDNA overexpressed in hybrid maize. The cDNA was reamplified and used to screen a corresponding cDNA library. A full length of a cDNA insert ZH01 was isolated and shown to have an open reading frame encoding 414a. a. Sequence comparison shows that ZH01 has extremely high similarity with the previously reported plant initiator factor elF-4A and distinct difference from another maize elF-4A gene MIF4A. Overexpression of ZH01 in hybrid may involve vigorous growth of hybrid maize seedlings.

Original language	English (US)
Pages (from-to)	282-286
Number of pages	5
Journal	Chinese Science Bulletin
Volume	43
Issue number	4
DOIs	https://doi.org/10.1007/bf02884536
State	Published - Feb 1998

Keywords

Heterosis
Initiation factor gene
Maize
mRNA differential display

ASJC Scopus subject areas

General

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10.1007/bf02884536

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title = "Isolation and cloning of a new gene encoding maize initiation factor",

abstract = "It was demonstrated that parental gene expression of maize in hybrid genetic background was different from that in their inbred lines. RT-PCR revealed a cDNA overexpressed in hybrid maize. The cDNA was reamplified and used to screen a corresponding cDNA library. A full length of a cDNA insert ZH01 was isolated and shown to have an open reading frame encoding 414a. a. Sequence comparison shows that ZH01 has extremely high similarity with the previously reported plant initiator factor elF-4A and distinct difference from another maize elF-4A gene MIF4A. Overexpression of ZH01 in hybrid may involve vigorous growth of hybrid maize seedlings.",

keywords = "Heterosis, Initiation factor gene, Maize, mRNA differential display",

author = "Jianguang Hu and Xiangshan Zhao and Ziqiang Yuan and Xiaoying Qian and Jinshui Yang",

note = "Funding Information: Acknowledgement This work was supported by the National Natural Science Foundation of China (Grant No. 19174029) and Climbing Project of China.",

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T1 - Isolation and cloning of a new gene encoding maize initiation factor

AU - Hu, Jianguang

AU - Zhao, Xiangshan

AU - Yuan, Ziqiang

AU - Qian, Xiaoying

AU - Yang, Jinshui

N1 - Funding Information: Acknowledgement This work was supported by the National Natural Science Foundation of China (Grant No. 19174029) and Climbing Project of China.

PY - 1998/2

Y1 - 1998/2

N2 - It was demonstrated that parental gene expression of maize in hybrid genetic background was different from that in their inbred lines. RT-PCR revealed a cDNA overexpressed in hybrid maize. The cDNA was reamplified and used to screen a corresponding cDNA library. A full length of a cDNA insert ZH01 was isolated and shown to have an open reading frame encoding 414a. a. Sequence comparison shows that ZH01 has extremely high similarity with the previously reported plant initiator factor elF-4A and distinct difference from another maize elF-4A gene MIF4A. Overexpression of ZH01 in hybrid may involve vigorous growth of hybrid maize seedlings.

AB - It was demonstrated that parental gene expression of maize in hybrid genetic background was different from that in their inbred lines. RT-PCR revealed a cDNA overexpressed in hybrid maize. The cDNA was reamplified and used to screen a corresponding cDNA library. A full length of a cDNA insert ZH01 was isolated and shown to have an open reading frame encoding 414a. a. Sequence comparison shows that ZH01 has extremely high similarity with the previously reported plant initiator factor elF-4A and distinct difference from another maize elF-4A gene MIF4A. Overexpression of ZH01 in hybrid may involve vigorous growth of hybrid maize seedlings.

KW - Heterosis

KW - Initiation factor gene

KW - Maize

KW - mRNA differential display

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Isolation and cloning of a new gene encoding maize initiation factor

Abstract

Keywords

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