Isolation and molecular characterization of a cax gene from Capsella bursa-pastoris

Juan Lin, Wen Zhang, Mingzhu Shi, Xinglong Wang, Xiaofen Sun, Kexuan Tang

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

A new cation exchangers (CAXs) gene was cloned and characterized from Capsella bursapastoris by rapid amplification of cDNA ends (RACE). The full-length cDNA sequence of cax from C. bursa-pastoris (designated as Cbcax51) was 1754 bp containing a 1398 bp open reading frame encoding a polypeptide of 466 amino-acid residues with a calculated molecular mass of 50.5 kDa and an isoelectric point of 5.69. The predicted CbCAX51 contained an IMP dehydrogenase/GMP reductase domain, two Na+/Ca2+ exchanger protein domains. Comparative and bioinformatics analyses revealed that CbCAX51 showed extensive homology with CAX from other plant species. The expression analysis by different treatments indicated that Cbcax51 could be activated by cold triggering and was related to the cold acclimation process, but its expression is regulated negatively by drought and not affected by ABA or salt.

Original languageEnglish (US)
Pages (from-to)229-235
Number of pages7
JournalBiocell
Volume32
Issue number3
StatePublished - 2008
Externally publishedYes

Keywords

  • Capsella bursa-pastoris
  • Cation exchangers (CAXs)
  • Freezing tolerance
  • RACE

ASJC Scopus subject areas

  • Cell Biology

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