Isolation and serial propagation of turkey rotaviruses in a fetal rhesus monkey kidney (MA104) cell line.

K. W. Theil, D. L. Reynolds, Y. M. Saif

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Turkey rotaviruses from the intestinal contents of poults were isolated and serially propagated in MA104 cell monolayers by a simple procedure. The initial virus isolation was done by low-speed centrifugation of the inoculum onto the monolayers, and subsequent passages were accomplished in roller-tube monolayers using trypsin-treated virus suspensions. Each of the turkey rotavirus isolates possessed the morphologic, antigenic, and genomic attributes characteristic of turkey group A rotaviruses. Attempts to isolate and serially propagate turkey rotavirus-like viruses in MA104 cell monolayers by this procedure were unsuccessful. Turkey reoviruses also did not serially propagate in MA104 cell monolayers by this procedure.

Original languageEnglish (US)
Pages (from-to)93-104
Number of pages12
JournalAvian diseases
Volume30
Issue number1
DOIs
StatePublished - 1986
Externally publishedYes

ASJC Scopus subject areas

  • Food Animals
  • Animal Science and Zoology
  • Immunology and Microbiology(all)

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