Isolation of a 2-picolinic acid-assimilating bacterium and its proposed degradation pathway

Chunli Zheng, Qiaorui Wang, Yanli Ning, Yurui Fan, Shanshan Feng, Chi He, Tian C. Zhang, Zhenxing Shen

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Burkholderia sp. ZD1, aerobically utilizes 2-picolinic acid as a source of carbon, nitrogen and energy, was isolated. ZD1 completely degraded 2-picolinic acid when the initial concentrations ranged from 25 to 300 mg/L. Specific growth rate (μ) and specific consumption rate (q) increased continually in the concentration range of 25–100 mg/L, and then declined. Based on the Haldane model and Andrew's model, μmax and qmax were calculated as 3.9 and 16.5 h−1, respectively. Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) was used to determine the main intermediates in the degradation pathway. Moreover, attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) was innovatively used to deduce the ring cleavage mechanism of N-heterocycle of 2-picolinic acid. To our knowledge, this is the first report on not only the utilization of 2-picolinic acid by a Burkholderia sp., but also applying FT-ICR-MS and ATR-FTIR for exploring the biodegradation pathway of organic compounds.

Original languageEnglish (US)
Pages (from-to)681-688
Number of pages8
JournalBioresource Technology
Volume245
DOIs
StatePublished - 2017

Keywords

  • 2-Picolinic acid
  • ATR-FTIR
  • Biodegradation pathway
  • Burkholderia sp.
  • FT-ICR-MS

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

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