Isolation of a heparan sulfate-containing proteoglycan from basement membrane.

J. R. Hassell, P. G. Robey, H. J. Barrach, J. Wilczek, S. I. Rennard, G. R. Martin

Research output: Contribution to journalArticle

474 Scopus citations

Abstract

We have isolated a unique, basement membrane proteoglycan from the Engelbreth-Holm-Swarm (EHS) sarcoma. This proteoglycan, estimated to be 0.75 X 10(6) daltons, was found to contain about equal amounts of protein and covalently linked heparan sulfate. Antibody prepared against this proteoglycan reacts with the basement membrane matrix in the tumor and with the basement membranes in skin, kidney, and cornea. These studies indicate that the heparan sulfate proteoglycan is a normal constituent of basement membranes that presumably plays an important role in the organization of basement membrane components and that also may determine the permeability of basement membranes to acidic molecules.

Original languageEnglish (US)
Pages (from-to)4494-4498
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume77
Issue number8
DOIs
StatePublished - Aug 1980

ASJC Scopus subject areas

  • General

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