Isolation of a novel orthobunyavirus from bat flies (Eucampsipoda africana)

Petrus Jansen Van Vuren; Michael R. Wiley; Gustavo Palacios; Nadia Storm; Wanda Markotter; Monica Birkhead; Alan Kemp; Janusz T. Paweska

doi:10.1099/jgv.0.000753

Isolation of a novel orthobunyavirus from bat flies (Eucampsipoda africana)

Petrus Jansen Van Vuren, Michael R. Wiley, Gustavo Palacios, Nadia Storm, Wanda Markotter, Monica Birkhead, Alan Kemp, Janusz T. Paweska

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

The Bunyaviridae family comprises viruses causing diseases of public and veterinary health importance, including viral haemorrhagic and arboviral fevers. We report the isolation, identification and genome characterization of a novel orthobunyavirus, named Wolkberg virus (WBV), from wingless bat fly ectoparasites (Eucampsipoda africana) of Egyptian fruit bats (Rousettus aegyptiacus) in South Africa. Complete genome sequence data of WBV suggests it is most closely related to two bat viruses (Mojuí dos Campos and Kaeng Khoi viruses) and an arbovirus (Nyando virus) previously shown to infect humans. WBV replicates to high titres in VeroE6 and C6-36 cells, characteristic of mosquito-borne arboviruses. These findings expand our knowledge of the diversity of orthobunyaviruses and their insect vector host range.

Original language	English (US)
Article number	000753
Pages (from-to)	935-945
Number of pages	11
Journal	Journal of General Virology
Volume	98
Issue number	5
DOIs	https://doi.org/10.1099/jgv.0.000753
State	Published - May 2017
Externally published	Yes

Keywords

Arbovirus
Bat flies
Bunyaviridae
Egyptian fruit bat
Eucampsipoda africana
Orthobunyavirus
Pathogen discovery
Rousettus aegyptiacus

ASJC Scopus subject areas

Virology

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10.1099/jgv.0.000753

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@article{cce30a52bb5443d1a21ba5b5a78a2311,

title = "Isolation of a novel orthobunyavirus from bat flies (Eucampsipoda africana)",

abstract = "The Bunyaviridae family comprises viruses causing diseases of public and veterinary health importance, including viral haemorrhagic and arboviral fevers. We report the isolation, identification and genome characterization of a novel orthobunyavirus, named Wolkberg virus (WBV), from wingless bat fly ectoparasites (Eucampsipoda africana) of Egyptian fruit bats (Rousettus aegyptiacus) in South Africa. Complete genome sequence data of WBV suggests it is most closely related to two bat viruses (Moju{\'i} dos Campos and Kaeng Khoi viruses) and an arbovirus (Nyando virus) previously shown to infect humans. WBV replicates to high titres in VeroE6 and C6-36 cells, characteristic of mosquito-borne arboviruses. These findings expand our knowledge of the diversity of orthobunyaviruses and their insect vector host range.",

keywords = "Arbovirus, Bat flies, Bunyaviridae, Egyptian fruit bat, Eucampsipoda africana, Orthobunyavirus, Pathogen discovery, Rousettus aegyptiacus",

author = "{Jansen Van Vuren}, Petrus and Wiley, {Michael R.} and Gustavo Palacios and Nadia Storm and Wanda Markotter and Monica Birkhead and Alan Kemp and Paweska, {Janusz T.}",

note = "Funding Information: The project is jointly funded by the following grants awarded to: Janusz T. Paweska (CDC Global Disease Detection program, GDD 5U19 GH000571-05/96667), Wanda Markotter (South African National Research Foundation, Grant UID 91496, 92524 and 98339; Poliomyelitis Research Foundation, PRF Grant number 12/14) and Petrus Jansen van Vuren (South African National Research Foundation, Incentive Funding for Rated Researchers, Grant UID 85544). The grant holders acknowledge that opinions, findings and conclusions or recommendations expressed in any publication generated by GDD and NRF supported research are those of the authors and that the GDD and NRF accept no liability whatsoever in this regard. Publisher Copyright: {\textcopyright} 2017 The Authors.",

year = "2017",

month = may,

doi = "10.1099/jgv.0.000753",

language = "English (US)",

volume = "98",

pages = "935--945",

journal = "Journal of General Virology",

issn = "0022-1317",

publisher = "Society for General Microbiology",

number = "5",

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T1 - Isolation of a novel orthobunyavirus from bat flies (Eucampsipoda africana)

AU - Jansen Van Vuren, Petrus

AU - Wiley, Michael R.

AU - Palacios, Gustavo

AU - Storm, Nadia

AU - Markotter, Wanda

AU - Birkhead, Monica

AU - Kemp, Alan

AU - Paweska, Janusz T.

N1 - Funding Information: The project is jointly funded by the following grants awarded to: Janusz T. Paweska (CDC Global Disease Detection program, GDD 5U19 GH000571-05/96667), Wanda Markotter (South African National Research Foundation, Grant UID 91496, 92524 and 98339; Poliomyelitis Research Foundation, PRF Grant number 12/14) and Petrus Jansen van Vuren (South African National Research Foundation, Incentive Funding for Rated Researchers, Grant UID 85544). The grant holders acknowledge that opinions, findings and conclusions or recommendations expressed in any publication generated by GDD and NRF supported research are those of the authors and that the GDD and NRF accept no liability whatsoever in this regard. Publisher Copyright: © 2017 The Authors.

PY - 2017/5

Y1 - 2017/5

N2 - The Bunyaviridae family comprises viruses causing diseases of public and veterinary health importance, including viral haemorrhagic and arboviral fevers. We report the isolation, identification and genome characterization of a novel orthobunyavirus, named Wolkberg virus (WBV), from wingless bat fly ectoparasites (Eucampsipoda africana) of Egyptian fruit bats (Rousettus aegyptiacus) in South Africa. Complete genome sequence data of WBV suggests it is most closely related to two bat viruses (Mojuí dos Campos and Kaeng Khoi viruses) and an arbovirus (Nyando virus) previously shown to infect humans. WBV replicates to high titres in VeroE6 and C6-36 cells, characteristic of mosquito-borne arboviruses. These findings expand our knowledge of the diversity of orthobunyaviruses and their insect vector host range.

AB - The Bunyaviridae family comprises viruses causing diseases of public and veterinary health importance, including viral haemorrhagic and arboviral fevers. We report the isolation, identification and genome characterization of a novel orthobunyavirus, named Wolkberg virus (WBV), from wingless bat fly ectoparasites (Eucampsipoda africana) of Egyptian fruit bats (Rousettus aegyptiacus) in South Africa. Complete genome sequence data of WBV suggests it is most closely related to two bat viruses (Mojuí dos Campos and Kaeng Khoi viruses) and an arbovirus (Nyando virus) previously shown to infect humans. WBV replicates to high titres in VeroE6 and C6-36 cells, characteristic of mosquito-borne arboviruses. These findings expand our knowledge of the diversity of orthobunyaviruses and their insect vector host range.

KW - Arbovirus

KW - Bat flies

KW - Bunyaviridae

KW - Egyptian fruit bat

KW - Eucampsipoda africana

KW - Orthobunyavirus

KW - Pathogen discovery

KW - Rousettus aegyptiacus

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ER -

Isolation of a novel orthobunyavirus from bat flies (Eucampsipoda africana)

Abstract

Keywords

ASJC Scopus subject areas

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