Isolation of Compounds with Antimutagenic Activity from Savoy Chieftain Cabbage

Terence Lawson, Janet Nunnallv, Betty Walker, Edward Bresnick, Desmond Wheeler, Maureen Wheeler

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15 Scopus citations


Dried leaves of Savoy Chieftain cabbage (Brassica oleracea) were extracted with methanol, methylene chloride, or petroleum ether. Soluble extracts were fractionated by flash chromatography on silica gel columns. Nonacosane, 15-nonacosanone, pheophytin a, and β-sitosterol were isolated and examined for their ability to inhibit the mutagenicity of N-methyl-N-nitrosourea (MNU) and 2-aminoanthracene (2-AA) in the Ames bacterial and V79 cell mammalian mutagenicity assays. In the Ames assay, nonacosane and pheophytin were without inhibitory action against either 2-AA- or MNU-induced mutagenesis. 15-Nonacosanone was more effective than 0-sitosterol. In the V79 assay, all four compounds were active against the mutagenicity of 2-AA but only nonacosane, 15-nonacosanone, and 0-sitosterol were active against that of MNU. Other crude extracts were identified with antimutagenic activity, but their compositions were not determined.

Original languageEnglish (US)
Pages (from-to)1363-1367
Number of pages5
JournalJournal of Agricultural and Food Chemistry
Issue number5
StatePublished - Sep 1 1989

ASJC Scopus subject areas

  • General Chemistry
  • General Agricultural and Biological Sciences


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