TY - JOUR
T1 - Isolation of Compounds with Antimutagenic Activity from Savoy Chieftain Cabbage
AU - Lawson, Terence
AU - Nunnallv, Janet
AU - Walker, Betty
AU - Bresnick, Edward
AU - Wheeler, Desmond
AU - Wheeler, Maureen
PY - 1989/9/1
Y1 - 1989/9/1
N2 - Dried leaves of Savoy Chieftain cabbage (Brassica oleracea) were extracted with methanol, methylene chloride, or petroleum ether. Soluble extracts were fractionated by flash chromatography on silica gel columns. Nonacosane, 15-nonacosanone, pheophytin a, and β-sitosterol were isolated and examined for their ability to inhibit the mutagenicity of N-methyl-N-nitrosourea (MNU) and 2-aminoanthracene (2-AA) in the Ames bacterial and V79 cell mammalian mutagenicity assays. In the Ames assay, nonacosane and pheophytin were without inhibitory action against either 2-AA- or MNU-induced mutagenesis. 15-Nonacosanone was more effective than 0-sitosterol. In the V79 assay, all four compounds were active against the mutagenicity of 2-AA but only nonacosane, 15-nonacosanone, and 0-sitosterol were active against that of MNU. Other crude extracts were identified with antimutagenic activity, but their compositions were not determined.
AB - Dried leaves of Savoy Chieftain cabbage (Brassica oleracea) were extracted with methanol, methylene chloride, or petroleum ether. Soluble extracts were fractionated by flash chromatography on silica gel columns. Nonacosane, 15-nonacosanone, pheophytin a, and β-sitosterol were isolated and examined for their ability to inhibit the mutagenicity of N-methyl-N-nitrosourea (MNU) and 2-aminoanthracene (2-AA) in the Ames bacterial and V79 cell mammalian mutagenicity assays. In the Ames assay, nonacosane and pheophytin were without inhibitory action against either 2-AA- or MNU-induced mutagenesis. 15-Nonacosanone was more effective than 0-sitosterol. In the V79 assay, all four compounds were active against the mutagenicity of 2-AA but only nonacosane, 15-nonacosanone, and 0-sitosterol were active against that of MNU. Other crude extracts were identified with antimutagenic activity, but their compositions were not determined.
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U2 - 10.1021/jf00089a034
DO - 10.1021/jf00089a034
M3 - Article
AN - SCOPUS:1542690959
SN - 0021-8561
VL - 37
SP - 1363
EP - 1367
JO - Journal of Agricultural and Food Chemistry
JF - Journal of Agricultural and Food Chemistry
IS - 5
ER -