Isolation of matrices from maize leaf nuclei: identification of a matrix-binding site adjacent to the Adh1 gene

Zoya Avramova, Jeffrey L. Bennetzen

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

Nuclear matrices were isolated from maize leaves by the two conventional methods usually employed for the preparation of the corresponding structures of animal origin. It is demonstrated that functionally competent matrices, recognizing and specifically binding the MAR-containing DNA of the mouse k-immunoglobulin gene may be prepared by both 2 M NaCl and LIS extractions of maize nuclei. A DNA region with a high affinity for the nuclear matrix was identified at the 5′ end of the maize Adh1-S gene, distal to the promoter region. The presence of sites of reported altered chromatin structure in this particular region is discussed. While the proximity and the cohabitation of MARs with different regulatory elements is a common feature of matrix association regions in animal systems, this is the first plant MAR identified in a region of known significance for gene regulation.

Original languageEnglish (US)
Pages (from-to)1135-1143
Number of pages9
JournalPlant Molecular Biology
Volume22
Issue number6
DOIs
StatePublished - Sep 1993

Keywords

  • Adh1 gene
  • MAR
  • maize
  • nuclear matrix

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

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