N2-[γ-L-(+)-Glutamyl]-carboxyphenylhydrazine (GCPH) has been isolated from Agaricus bisporus, the cultivated mushroom of commerce of the Western hemisphere. The compound was purified by using a combination of cation-exchange and reverse-phase high performance liquid chromatography and the structure confirmed by using fast-atom bombardment (FAB) mass spectrometry and FAB/tandem mass spectrometry. The average level of GCPH in the four samples analyzed was 42 ± 3 μg/g of wet mushroom.
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)