Isotope effect on the carcinogenicity of 3-methylcholanthrene in mouse skin by selective deuteration of the 1-methylene group: Biological evidence for a mechanism of tumor initiation

E. Cavalieri, H. Garcia, P. Mailander, K. Patil

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

3-MethylchoIanthrene (MC) in chloroform and trifluoroacetic acid-d1 yielded 3-methylcholanthrene-d4 (MC-d4), a compound selectively deuterated at the 1-, 5-, and 6-carbon atoms. In turn, the protodedeuteration of the labeled hydrocarbon at the 6-carbon atom led to 3-methyleholanthrene-d3 (MC-d3). A comparative test for carcinogenicity between MC and MC-d3 by repeated skin painting on female Swiss mice showed a significantly lower tumorigenic activity of the latter. The result implies that the 1-carbon atom of the hydrocarbon is a critical binding-site to cellular targets in the tumor-initiating process.

Original languageEnglish (US)
Pages (from-to)179-189
Number of pages11
JournalChemico-Biological Interactions
Volume11
Issue number3
DOIs
StatePublished - Sep 1975

ASJC Scopus subject areas

  • Toxicology

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