ITRAQ analysis with paul ion trap-obstacle solved

Anna Drabik, Anna Bodzoń-Kułakowska, Piotr Suder, Paweł Ciborowski, Jerzy Silberring

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Mass spectrometers equipped with ion trap analyzers have been significantly improved due to their high performance and wide application area accompanying the low costs of purchase. Despite several advantages, such as reasonable resolution at low cost, high sensitivity, and capability for multistage analysis, ion traps have an important drawback: low mass cutoff during tandem mass spectrometry analysis MSn. Although the low mass cutoff associated with the ion trap does not seriously obstruct peptide identification, it may cause a serious problem in identification of small molecules (posttranslational modifications, e.g., glycan structures) and quantification of peptides with multiplexed isobaric tag reagents. The presented approach offers the possibility to use isobaric tags for relative and absolute quantification labeling (iTRAQ) for quantitative, proteomic analysis using typical, widely available ion trap devices and manufacturer's software. We have performed series of analyses of standard protein labeled with isobaric tags in various concentration ratios to prove quantitative capabilities of this approach.

Original languageEnglish (US)
Pages (from-to)4607-4611
Number of pages5
JournalJournal of proteome research
Volume12
Issue number10
DOIs
StatePublished - Oct 4 2013

Keywords

  • iTRAQ
  • ion trap
  • quantitative proteomics

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

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    Drabik, A., Bodzoń-Kułakowska, A., Suder, P., Ciborowski, P., & Silberring, J. (2013). ITRAQ analysis with paul ion trap-obstacle solved. Journal of proteome research, 12(10), 4607-4611. https://doi.org/10.1021/pr400316x