Japanese encephalitis virus immunoglobulin M antibodies in porcine sera.

D. S. Burke, M. Tingpalapong, M. R. Elwell, P. S. Paul, R. A. Van Deusen

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

A solid-phase enzyme-linked immunosorbent assay (ELISA) was developed for detection of porcine immunoglobulin (Ig)M antibodies to Japanese encephalitis virus (JEV). Antibodies in sera were captured onto the solid phase of Microtiter plates sensitized with mouse monoclonal antibodies to porcine mu heavy chain. Virus antigen binding to the lawn of IgM was quantitated by subsequent binding of peroxidase-labeled human hyperimmune anti-JEV IgG, which in the final step, catalyzed a substrate color change. In sucrose density-gradient fractionated sera from recently infected pigs, the peak of ELISA JEV IgM activity corresponded to the peak of 18-S, 2-mercaptoethanol-sensitive hemagglutination-inhibiting (HAI) antibody activity. Within 2 to 3 days, JEV-infected sentinel pigs developed high JEV IgM activity; this activity decreased within 2 weeks. Among specimens collected from 99 random swine at abattoirs in Thailand during a period of low JEV transmission, none of 25 JEV HAI-negative sera had JEV IgM activity, 7 of 74 JEV HAI-positive sera did have JEV IgM activity, and the remaining 67 sera had readily detectable JEV HAI antibodies, but lacked JEV IgM. The JEV IgM solid-phase ELISA was useful for rapidly diagnosing active or recent JEV infections in swine.

Original languageEnglish (US)
Pages (from-to)2054-2057
Number of pages4
JournalAmerican journal of veterinary research
Volume46
Issue number10
StatePublished - Oct 1 1985

ASJC Scopus subject areas

  • veterinary(all)

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    Burke, D. S., Tingpalapong, M., Elwell, M. R., Paul, P. S., & Van Deusen, R. A. (1985). Japanese encephalitis virus immunoglobulin M antibodies in porcine sera. American journal of veterinary research, 46(10), 2054-2057.