Jhe in Gryllus assimilis: Cloning, sequence-activity associations and phylogeny

E. J. Crone, A. J. Zera, A. Anand, J. G. Oakeshott, T. D. Sutherland, R. J. Russell, L. G. Harshman, F. G. Hoffmann, C. Claudianos

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

The 458 amino acid sequence of a mature JHE protein from the cricket Gryllus assimilis was identified after isolating the partial cDNA sequence encoding this protein from a fat body and midgut cDNA library. This hemimetabolan JHE sequence shows over 40% amino acid similarity to the known JHE sequences of several holometabolous insects. It also includes previously determined peptide sequences for G. assimilis JHE as well as two other motifs associated with JHE enzymes in holometabolous insects. The predicted molecular weight of the protein agrees with that of the JHE previously purified from G. assimilis. Partial genomic sequence encoding the Jhe contains two large (1330 and 2918 bp) introns. No coding DNA sequence variation was observed over a 1293 bp region between selected lines differing six to eight-fold in hemolymph JHE activity. However, a 19 bp indel was found in one of the introns; the insertion was strongly associated with elevated hemolymph activity, both in the selected lines and in the F2 progeny of crosses between them. Phylogenetic analyses localised the G. assimilis JHE to a clade containing dipteran and coleopteran JHEs, with lepidopteran JHEs occurring in a separate clade.

Original languageEnglish (US)
Pages (from-to)1359-1365
Number of pages7
JournalInsect Biochemistry and Molecular Biology
Volume37
Issue number12
DOIs
StatePublished - Dec 2007

Keywords

  • Gryllus assimilis
  • Hemimetabolous insect
  • Indel polymorphism
  • Juvenile hormone esterase gene
  • Phylogeny

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Insect Science

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