Juxtacrine activation of EGFR regulates claudin expression and increases transepithelial resistance

Amar B. Singh, Keisuke Sugimoto, Punita Dhawan, Raymond C. Harris

Research output: Contribution to journalArticlepeer-review

39 Scopus citations


Heparin-binding (HB)-EGF, a ligand for EGF receptors, is synthesized as a membrane-anchored precursor that is potentially capable of juxtacrine activation of EGF receptors. However, the physiological importance of such juxtacrine signaling remains poorly described, due to frequent inability to distinguish effects mediated by membrane-anchored HB-EGF vs. mature "secreted HB-EGF." In our studies, using stable expression of a noncleavable, membrane-anchored rat HB-EGF isoform (MDCKrat5aa cells) in Madin-Darby canine kidney (MDCK) II cells, we observed a significant increase in transepithelial resistance (TER). Similar significant increases in TER were observed on stable expression of an analogous, noncleavable, membrane-anchored human HB-EGF construct (MDCKhuman5aa cells). The presence of noncleavable, membrane-anchored HB-EGF led to alterations in the expression of selected claudin family members, including a marked decrease in claudin-2 in MDCKrat5aa cells compared with the control MDCK cells. Reexpression of claudin-2 in MDCKrat5aa cells largely prevented the increases in TER. Ion substitution studies indicated decreased paracellular ionic permeability of Na+ in MDCKrat5aa cells, further indicating that the altered claudin-2 expression mediated the increased TER seen in these cells. In a Ca2+-switch model, increased phosphorylation of EGF receptor and Akt was observed in MDCKrat5aa cells compared with the control MDCK cells, and inhibition of these pathways inhibited TER changes specifically in MDCKrat5aa cells. Therefore, we hypothesize that juxtacrine activation of EGFR by membrane-anchored HB-EGF may play an important role in the regulation of tight junction proteins and TER.

Original languageEnglish (US)
Pages (from-to)C1660-C1668
JournalAmerican Journal of Physiology - Cell Physiology
Issue number5
StatePublished - Nov 2007
Externally publishedYes


  • Epidermal growth factor receptor
  • Heparin-binding-epidermal growth factor
  • Membrane-anchored

ASJC Scopus subject areas

  • Physiology
  • Cell Biology


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