Keratinocyte-derived T cell costimulation induces preferential production of IL-2 and IL-4 but not IFN-γ

Richard E. Goodman, Frank Nestle, Yathi M. Naidu, Jonathan M. Green, Craig B. Thompson, Brian J. Nickoloff, Laurence A. Turka

Research output: Contribution to journalArticlepeer-review

76 Scopus citations

Abstract

By using superantigens, we have found previously that keratinocytes activated by IFN-γ could serve as accessory cells, providing costimulatory signals needed to induce T cell proliferation. Here, we compared the profile of cytokines produced by T cells stimulated in the presence of activated keratinocytes with the response seen using professional APCs. When keratinocytes are used as accessory cells there is a specific defect in T cell IFN-γ production, whereas IL-2 and IL-4 are induced at levels comparable with those seen when professional APCs are used as accessory cells. Because keratinocytes express BB-1, a CD28-ligand distinct from B7-1 or B7-2 (which are found on professional APCs), we examined the possibility that the defect in IFN-γ production might be a result of nonproductive CD28 engagement. However, even when the CD28 pathway is directly activated by a stimulatory mAb, there is no induction of IFN-γ production in keratinocyte- supported cultures. In these same cultures IL-2 production is increased 10- fold, thus demonstrating a specific deficiency in the induction of IFN-γ rather than a failure to respond to CD28 stimulation. Analysis by reverse transcriptase-PCR and ELISA for the inducible p40 chain of IL-12 reveals that keratinocytes produce little if any messenger RNA and no protein for IL-12 p40 compared with professional APCs. Addition of rIL-12 to keratinocyte- supported cultures restores IFN-γ levels to those seen when professional APCs are present. Finally, when T cells are restimulated and analyzed at later time points (10 to 14 days) we find a refinement in cytokine profiles: T cells stimulated in the presence of professional APCs produced the Th1 cytokines IL-2 and IFN-γ, whereas T cells stimulated in the presence of activated keratinocytes produced only the Th2 cytokine IL-4. The specific ability of keratinocytes to induce a Th2 response seems most closely linked to their absence of IL-12 production, and may be important in the maintenance of peripheral tolerance to self-Ags or in the immune response to exogenous Ags, pathogens, or haptens encountered in skin.

Original languageEnglish (US)
Pages (from-to)5189-5198
Number of pages10
JournalJournal of Immunology
Volume152
Issue number11
StatePublished - Jun 1 1994

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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