Ketamine inhibits lipopolysacharide (LPS) induced gastric luminal fluid accumulation

James W. Suliburk, Ernest A. Gonzalez, Stacey D. Moore-Olufemi, Norman Weisbrodt, Frederick A. Moore, David W. Mercer

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Introduction. Lipopolysacharide (LPS) causes gastrointestinal ileus and gastric luminal fluid accumulation. Ketamine, an anti-inflammatory anesthetic agent attenuates accumulation of luminal fluid. However, its effects on gastrointestinal transit induced by endotoxemia are unknown. The purpose of this study was to determine if the anti-inflammatory properties of ketamine improve impaired gastric emptying and gastrointestinal transit because of LPS. Materials and methods. Rats were given ketamine (70 mg/kg i.p.) or saline 1 h before LPS (20 mg/kg, i.p.) or saline injection. Five hours after LPS injection, rats were gavaged with 1 cc consisting of 0.1 ml of 5 mm FITC Dextran added to 0.9 ml of saline. After 30 min, rats were sacrificed, and gastric emptying, gastrointestinal transit, and gastric fluid accumulation determined. Gastric and ileal mucosa were harvested for analysis of inducible nitric oxide synthase (iNOS) (Western immunoblot). Results are reported as mean ± SE (n < 5 per group; ANOVA). Results. Ketamine did not prevent LPS induced gastrointestinal ileus, nor did it improve gastric emptying. More importantly, it did not worsen gastrointestinal function or gastric emptying when compared to saline controls. However, it did decrease LPS induced gastric luminal fluid accumulation and blunted iNOS expression in both the stomach and ileum. Conclusion. These data indicate that the ability of ketamine to attenuate gastric fluid accumulation is not because of improved gastric emptying or improved gastrointestinal transit. Moreover, while iNOS may play a role in LPS induced gastric luminal fluid accumulation, it does not appear to be a major mediator of the gastrointestinal ileus caused by LPS.

Original languageEnglish (US)
Pages (from-to)203-207
Number of pages5
JournalJournal of Surgical Research
Volume127
Issue number2
DOIs
StatePublished - Aug 1 2005

Keywords

  • Anesthetics
  • Gastric
  • Ileum
  • Ileus
  • Ketamine
  • LPS
  • Lipopolysacharide
  • Nitric oxide
  • Transit
  • iNOS

ASJC Scopus subject areas

  • Surgery

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