Kinetic analysis of drug-protein interactions by affinity chromatography

Cong Bi, Sandya Beeram, Zhao Li, Xiwei Zheng, David S. Hage

Research output: Contribution to journalReview article

8 Scopus citations

Abstract

Information on the kinetics of drug-protein interactions is of crucial importance in drug discovery and development. Several methods based on affinity chromatography have been developed in recent years to examine the association and dissociation rates of these processes. These techniques include band-broadening measurements, the peak decay method, peak fitting methods, the split-peak method, and free fraction analysis. This review will examine the general principles and applications of these approaches and discuss their use in the characterization, screening and analysis of drug-protein interactions in the body.

Original languageEnglish (US)
Pages (from-to)16-21
Number of pages6
JournalDrug Discovery Today: Technologies
Volume17
DOIs
StatePublished - Oct 1 2015

ASJC Scopus subject areas

  • Molecular Medicine
  • Drug Discovery

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