The kinetics of oxygen and CO binding by the high molecular weight hemoglobin (ca. 3,000,000) from the annelid Cirraformia grandis have been measured by stopped-flow and flash-photolysis as a function of pH. Flash-photolysis studies of the Hb* form suggest weak heme-heme interaction for ligand binding in the ferrous state. The rate constant for oxygen dissociation increases by a factor of ca. 800 from pH 9 to pH 6. Rate constants for oxygen and CO association are virtually unchanged in the range pH 6-9.
|Original language||English (US)|
|Number of pages||7|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Jun 19 1973|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology