Kinetics of diversification of bovine primary b-cell repertoire

M. Lucier, Y. K. Jeong, R. Thompson, R. A. Goldsbv, B. A. Osborne

Research output: Contribution to journalArticlepeer-review

Abstract

SSCP and sequencing have been used to examine the establishment and diversification of the bovine primary Ig repertoire. Evidence of B-cells in fetal liver and ileum is found as early as 110 days of gestation. The same light chain gene sequence is expressed in both intestine and liver. Therefore, although light chain genes have undergone rearrangement, significant diversification of the light chain repertoire has not yet begun. In newborns, sequences of light chain genes expressed in individual IFF follicles taken at 2 hours, 6 days, 12 days, 24 days and 47 days revealed little change in the extent of diversity found within a follicle. The light chain diversity of blood rises from a modest level of diversity (11 repeats in 16 sequences) at birth to almost total diversity (only 2 repeats in 16 sequences at 10 weeks. In order to determine if the IPP contains a B cell population that is phenotypically distinct from the peripheral B-cell population, monoclonal antibodies were raised against IPP B-cells. Some of these antibodies were specific for IPP B-cells and failed to react with peripheral B-Cells. Furthermore, some of the IPPspecific mABs only recognized a subpopulation of IPP B-cells. These results indicated that the IPP B-cells are a developmentally distinct population of B-cells that contains distinctive subsets. This work was supported by grants from NSF (MCB9405257) and NIH (GMRO136344).

Original languageEnglish (US)
Pages (from-to)A1169
JournalFASEB Journal
Volume10
Issue number6
StatePublished - 1996

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

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