TY - GEN
T1 - Kinetics of enzymatic hydrolysis revealed by video rate AFM single molecule analysis
AU - Song, Bo
AU - Xi, Ning
AU - Sun, Zhiyong
AU - Yang, Ruiguo
AU - Chen, Liangliang
AU - Zhou, Zhanxin
AU - Yang, Yongliang
AU - Du, Wenjian
AU - Xi, Jun
N1 - Publisher Copyright:
© 2015 IEEE.
PY - 2015
Y1 - 2015
N2 - The rapid growing bio-refining industry is hindered by the slow reaction rate of cellobiohydrolase catalyzed hydrolysis. Analysis of the mechanism of enzyme interaction in the cellobiohydrolase catalyzed hydrolysis is difficult because of the variety factors affecting the enzymatic reaction rate. In this study, we evaluated the processive hydrolysis parameters in the molecular level using a video rate atomic force microscopy (AFM). Both dissociate and association rates (koff and kon) have been calculated by an experimental data fitted Michaelis-Menten model and real-time observation experimental data. The difference in these two association rates suggested that the cellulose substrate governs the hydrolysis rate and that can deepen the understanding of the mechanisms of processive hydrolysis.
AB - The rapid growing bio-refining industry is hindered by the slow reaction rate of cellobiohydrolase catalyzed hydrolysis. Analysis of the mechanism of enzyme interaction in the cellobiohydrolase catalyzed hydrolysis is difficult because of the variety factors affecting the enzymatic reaction rate. In this study, we evaluated the processive hydrolysis parameters in the molecular level using a video rate atomic force microscopy (AFM). Both dissociate and association rates (koff and kon) have been calculated by an experimental data fitted Michaelis-Menten model and real-time observation experimental data. The difference in these two association rates suggested that the cellulose substrate governs the hydrolysis rate and that can deepen the understanding of the mechanisms of processive hydrolysis.
UR - http://www.scopus.com/inward/record.url?scp=84964355798&partnerID=8YFLogxK
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U2 - 10.1109/NANO.2015.7388868
DO - 10.1109/NANO.2015.7388868
M3 - Conference contribution
AN - SCOPUS:84964355798
T3 - IEEE-NANO 2015 - 15th International Conference on Nanotechnology
SP - 1288
EP - 1291
BT - IEEE-NANO 2015 - 15th International Conference on Nanotechnology
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 15th IEEE International Conference on Nanotechnology, IEEE-NANO 2015
Y2 - 27 July 2015 through 30 July 2015
ER -