Kinetics of enzymatic hydrolysis revealed by video rate AFM single molecule analysis

Bo Song; Ning Xi; Zhiyong Sun; Ruiguo Yang; Liangliang Chen; Zhanxin Zhou; Yongliang Yang; Wenjian Du; Jun Xi

doi:10.1109/NANO.2015.7388868

Kinetics of enzymatic hydrolysis revealed by video rate AFM single molecule analysis

Bo Song, Ning Xi, Zhiyong Sun, Ruiguo Yang, Liangliang Chen, Zhanxin Zhou, Yongliang Yang, Wenjian Du, Jun Xi

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

The rapid growing bio-refining industry is hindered by the slow reaction rate of cellobiohydrolase catalyzed hydrolysis. Analysis of the mechanism of enzyme interaction in the cellobiohydrolase catalyzed hydrolysis is difficult because of the variety factors affecting the enzymatic reaction rate. In this study, we evaluated the processive hydrolysis parameters in the molecular level using a video rate atomic force microscopy (AFM). Both dissociate and association rates (koff and kon) have been calculated by an experimental data fitted Michaelis-Menten model and real-time observation experimental data. The difference in these two association rates suggested that the cellulose substrate governs the hydrolysis rate and that can deepen the understanding of the mechanisms of processive hydrolysis.

Original language	English (US)
Title of host publication	IEEE-NANO 2015 - 15th International Conference on Nanotechnology
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	1288-1291
Number of pages	4
ISBN (Electronic)	9781467381550
DOIs	https://doi.org/10.1109/NANO.2015.7388868
State	Published - 2015
Externally published	Yes
Event	15th IEEE International Conference on Nanotechnology, IEEE-NANO 2015 - Rome, Italy Duration: Jul 27 2015 → Jul 30 2015

Publication series

Name	IEEE-NANO 2015 - 15th International Conference on Nanotechnology

Other

Other	15th IEEE International Conference on Nanotechnology, IEEE-NANO 2015
Country	Italy
City	Rome
Period	7/27/15 → 7/30/15

ASJC Scopus subject areas

Process Chemistry and Technology
Electrical and Electronic Engineering
Ceramics and Composites
Electronic, Optical and Magnetic Materials
Surfaces, Coatings and Films

Access to Document

10.1109/NANO.2015.7388868

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Cite this

Song, B., Xi, N., Sun, Z., Yang, R., Chen, L., Zhou, Z., Yang, Y., Du, W., & Xi, J. (2015). Kinetics of enzymatic hydrolysis revealed by video rate AFM single molecule analysis. In IEEE-NANO 2015 - 15th International Conference on Nanotechnology (pp. 1288-1291). [7388868] (IEEE-NANO 2015 - 15th International Conference on Nanotechnology). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/NANO.2015.7388868

Kinetics of enzymatic hydrolysis revealed by video rate AFM single molecule analysis. / Song, Bo; Xi, Ning; Sun, Zhiyong; Yang, Ruiguo; Chen, Liangliang; Zhou, Zhanxin; Yang, Yongliang; Du, Wenjian; Xi, Jun.

IEEE-NANO 2015 - 15th International Conference on Nanotechnology. Institute of Electrical and Electronics Engineers Inc., 2015. p. 1288-1291 7388868 (IEEE-NANO 2015 - 15th International Conference on Nanotechnology).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Song, B, Xi, N, Sun, Z, Yang, R, Chen, L, Zhou, Z, Yang, Y, Du, W & Xi, J 2015, Kinetics of enzymatic hydrolysis revealed by video rate AFM single molecule analysis. in IEEE-NANO 2015 - 15th International Conference on Nanotechnology., 7388868, IEEE-NANO 2015 - 15th International Conference on Nanotechnology, Institute of Electrical and Electronics Engineers Inc., pp. 1288-1291, 15th IEEE International Conference on Nanotechnology, IEEE-NANO 2015, Rome, Italy, 7/27/15. https://doi.org/10.1109/NANO.2015.7388868

Song B, Xi N, Sun Z, Yang R, Chen L, Zhou Z et al. Kinetics of enzymatic hydrolysis revealed by video rate AFM single molecule analysis. In IEEE-NANO 2015 - 15th International Conference on Nanotechnology. Institute of Electrical and Electronics Engineers Inc. 2015. p. 1288-1291. 7388868. (IEEE-NANO 2015 - 15th International Conference on Nanotechnology). https://doi.org/10.1109/NANO.2015.7388868

Song, Bo ; Xi, Ning ; Sun, Zhiyong ; Yang, Ruiguo ; Chen, Liangliang ; Zhou, Zhanxin ; Yang, Yongliang ; Du, Wenjian ; Xi, Jun. / Kinetics of enzymatic hydrolysis revealed by video rate AFM single molecule analysis. IEEE-NANO 2015 - 15th International Conference on Nanotechnology. Institute of Electrical and Electronics Engineers Inc., 2015. pp. 1288-1291 (IEEE-NANO 2015 - 15th International Conference on Nanotechnology).

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abstract = "The rapid growing bio-refining industry is hindered by the slow reaction rate of cellobiohydrolase catalyzed hydrolysis. Analysis of the mechanism of enzyme interaction in the cellobiohydrolase catalyzed hydrolysis is difficult because of the variety factors affecting the enzymatic reaction rate. In this study, we evaluated the processive hydrolysis parameters in the molecular level using a video rate atomic force microscopy (AFM). Both dissociate and association rates (koff and kon) have been calculated by an experimental data fitted Michaelis-Menten model and real-time observation experimental data. The difference in these two association rates suggested that the cellulose substrate governs the hydrolysis rate and that can deepen the understanding of the mechanisms of processive hydrolysis.",

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AB - The rapid growing bio-refining industry is hindered by the slow reaction rate of cellobiohydrolase catalyzed hydrolysis. Analysis of the mechanism of enzyme interaction in the cellobiohydrolase catalyzed hydrolysis is difficult because of the variety factors affecting the enzymatic reaction rate. In this study, we evaluated the processive hydrolysis parameters in the molecular level using a video rate atomic force microscopy (AFM). Both dissociate and association rates (koff and kon) have been calculated by an experimental data fitted Michaelis-Menten model and real-time observation experimental data. The difference in these two association rates suggested that the cellulose substrate governs the hydrolysis rate and that can deepen the understanding of the mechanisms of processive hydrolysis.

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