l-[3H]Glutamate binds to kainate-, NMDA- and AMPA-sensitive binding sites: an autoradiographic analysis

Daniel T. Monaghan, Deborah Yao, Carl W. Cotman

Research output: Contribution to journalArticlepeer-review

149 Scopus citations

Abstract

The anatomical distribution of l-[3H]glutamate binding sites was determined in the presence of various glutamate analogues using quantitative autoradiography. The binding of l-[3H]glutamate is accounted for the presence of 3 distinct binding sites when measured in the absence of Ca2+, Cl- and Na+ ions. The anatomical distribution and pharmacological specificity of these binding sites correspond to that reported for the 3 excitatory amino acid binding sites selectively labeled by d-[3H]2-amino-5-phosphonopentanoate (d-[3H]AP5), [3H]kainate ([3H]KA) and [3H]α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid ([3H]AMPA) which are thought to be selective ligands for the N-methyl-d-aspartate (NMDA), KA and quisqualate (QA) receptors, respectively.

Original languageEnglish (US)
Pages (from-to)378-383
Number of pages6
JournalBrain Research
Volume340
Issue number2
DOIs
StatePublished - Aug 12 1985

Keywords

  • N-methyl-d-aspartate (NMDA)
  • autoradiography
  • glutamate binding site
  • glutamate receptor
  • kainate
  • α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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