l-[3H]Glutamate binds to kainate-, NMDA- and AMPA-sensitive binding sites: an autoradiographic analysis

Daniel T. Monaghan; Deborah Yao; Carl W. Cotman

doi:10.1016/0006-8993(85)90936-9

l-[³H]Glutamate binds to kainate-, NMDA- and AMPA-sensitive binding sites: an autoradiographic analysis

Daniel T. Monaghan, Deborah Yao, Carl W. Cotman

Research output: Contribution to journal › Article › peer-review

159 Scopus citations

Abstract

The anatomical distribution of l-[³H]glutamate binding sites was determined in the presence of various glutamate analogues using quantitative autoradiography. The binding of l-[³H]glutamate is accounted for the presence of 3 distinct binding sites when measured in the absence of Ca²⁺, Cl^- and Na⁺ ions. The anatomical distribution and pharmacological specificity of these binding sites correspond to that reported for the 3 excitatory amino acid binding sites selectively labeled by d-[³H]2-amino-5-phosphonopentanoate (d-[³H]AP5), [³H]kainate ([³H]KA) and [³H]α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid ([³H]AMPA) which are thought to be selective ligands for the N-methyl-d-aspartate (NMDA), KA and quisqualate (QA) receptors, respectively.

Original language	English (US)
Pages (from-to)	378-383
Number of pages	6
Journal	Brain Research
Volume	340
Issue number	2
DOIs	https://doi.org/10.1016/0006-8993(85)90936-9
State	Published - Aug 12 1985
Externally published	Yes

Keywords

N-methyl-d-aspartate (NMDA)
autoradiography
glutamate binding site
glutamate receptor
kainate
α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)

ASJC Scopus subject areas

General Neuroscience
Molecular Biology
Clinical Neurology
Developmental Biology

Access to Document

10.1016/0006-8993(85)90936-9

Cite this

@article{ade6204c713f44a596a987f72862adca,

title = "l-[3H]Glutamate binds to kainate-, NMDA- and AMPA-sensitive binding sites: an autoradiographic analysis",

abstract = "The anatomical distribution of l-[3H]glutamate binding sites was determined in the presence of various glutamate analogues using quantitative autoradiography. The binding of l-[3H]glutamate is accounted for the presence of 3 distinct binding sites when measured in the absence of Ca2+, Cl- and Na+ ions. The anatomical distribution and pharmacological specificity of these binding sites correspond to that reported for the 3 excitatory amino acid binding sites selectively labeled by d-[3H]2-amino-5-phosphonopentanoate (d-[3H]AP5), [3H]kainate ([3H]KA) and [3H]α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid ([3H]AMPA) which are thought to be selective ligands for the N-methyl-d-aspartate (NMDA), KA and quisqualate (QA) receptors, respectively.",

keywords = "N-methyl-d-aspartate (NMDA), autoradiography, glutamate binding site, glutamate receptor, kainate, α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)",

author = "Monaghan, {Daniel T.} and Deborah Yao and Cotman, {Carl W.}",

note = "Funding Information: We would like to thank Darryl Toy for technical assistance. This work was supported by ARO Grant DAAG 29-82-K-0194 and NIH Grant RRO t192 (image analysis equipment).",

year = "1985",

month = aug,

day = "12",

doi = "10.1016/0006-8993(85)90936-9",

language = "English (US)",

volume = "340",

pages = "378--383",

journal = "Brain Research",

issn = "0006-8993",

publisher = "Elsevier B.V.",

number = "2",

}

TY - JOUR

T1 - l-[3H]Glutamate binds to kainate-, NMDA- and AMPA-sensitive binding sites

T2 - an autoradiographic analysis

AU - Monaghan, Daniel T.

AU - Yao, Deborah

AU - Cotman, Carl W.

N1 - Funding Information: We would like to thank Darryl Toy for technical assistance. This work was supported by ARO Grant DAAG 29-82-K-0194 and NIH Grant RRO t192 (image analysis equipment).

PY - 1985/8/12

Y1 - 1985/8/12

N2 - The anatomical distribution of l-[3H]glutamate binding sites was determined in the presence of various glutamate analogues using quantitative autoradiography. The binding of l-[3H]glutamate is accounted for the presence of 3 distinct binding sites when measured in the absence of Ca2+, Cl- and Na+ ions. The anatomical distribution and pharmacological specificity of these binding sites correspond to that reported for the 3 excitatory amino acid binding sites selectively labeled by d-[3H]2-amino-5-phosphonopentanoate (d-[3H]AP5), [3H]kainate ([3H]KA) and [3H]α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid ([3H]AMPA) which are thought to be selective ligands for the N-methyl-d-aspartate (NMDA), KA and quisqualate (QA) receptors, respectively.

AB - The anatomical distribution of l-[3H]glutamate binding sites was determined in the presence of various glutamate analogues using quantitative autoradiography. The binding of l-[3H]glutamate is accounted for the presence of 3 distinct binding sites when measured in the absence of Ca2+, Cl- and Na+ ions. The anatomical distribution and pharmacological specificity of these binding sites correspond to that reported for the 3 excitatory amino acid binding sites selectively labeled by d-[3H]2-amino-5-phosphonopentanoate (d-[3H]AP5), [3H]kainate ([3H]KA) and [3H]α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid ([3H]AMPA) which are thought to be selective ligands for the N-methyl-d-aspartate (NMDA), KA and quisqualate (QA) receptors, respectively.

KW - N-methyl-d-aspartate (NMDA)

KW - autoradiography

KW - glutamate binding site

KW - glutamate receptor

KW - kainate

KW - α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)

UR - http://www.scopus.com/inward/record.url?scp=0022270479&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022270479&partnerID=8YFLogxK

U2 - 10.1016/0006-8993(85)90936-9

DO - 10.1016/0006-8993(85)90936-9

M3 - Article

C2 - 2862960

AN - SCOPUS:0022270479

SN - 0006-8993

VL - 340

SP - 378

EP - 383

JO - Brain Research

JF - Brain Research

IS - 2

ER -