Laminar flow cells for single-molecule studies of DNA-protein interactions

Laurence R. Brewer, Piero R. Bianco

Research output: Contribution to journalReview articlepeer-review

82 Scopus citations


Microfluidic flow cells are used in single-molecule experiments, enabling measurements to be made with high spatial and temporal resolution. We discuss the fundamental processes affecting flow cell operation and describe the flow cells in use at present for studying the interaction of optically trapped or mechanically isolated, single DNA molecules with proteins. To assist the experimentalist in flow cell selection, we review the construction techniques and materials used to fabricate both single- and multiple-channel flow cells and the advantages of each design for different experiments.

Original languageEnglish (US)
Pages (from-to)517-525
Number of pages9
JournalNature Methods
Issue number6
StatePublished - Jun 2008
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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